Identification of mammalian sperm surface antigens: II. Characterization of an acrosomal cap protein and a tail protein using monoclonal anti-mouse sperm antibodies

Eli D. Schmell, Bela J. Gulyas, Lydia C. Yuan, Thomas August

Research output: Contribution to journalArticle

Abstract

Monoclonal anti-mouse sperm antibodies have been produced by fusing mouse myeloma cells with spleen cells from rats immunized with epididymal sperm of C3H mice. Immunoprecipitation and immunoperoxidase techniques showed that one such monoclonal antibody, AMS IV-33, recognized a 200 000 dalton protein localized on the acrosomal cap of the sperm cell. Two other monoclonal antibodies AMS IV-54 and -76, reacted with a 68 000 dalton component on the surface of the sperm tail. Both antigenic targets were species specific and were present in about equal amounts on sperm from several different strains of mice. The tail protein was sperm specific, whereas the antibody reacting with the acrosomal cap protein also appeared to react somewhat with antigens present in other mouse tissues.

Original languageEnglish (US)
Pages (from-to)91-106
Number of pages16
JournalJournal of Reproductive Immunology
Volume4
Issue number2
DOIs
StatePublished - 1982

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Surface Antigens
Spermatozoa
Tail
Antibodies
Proteins
Monoclonal Antibodies
Sperm Tail
Inbred C3H Mouse
Immunoenzyme Techniques
Immunoprecipitation
Spleen
Antigens

ASJC Scopus subject areas

  • Immunology
  • Reproductive Medicine

Cite this

Identification of mammalian sperm surface antigens : II. Characterization of an acrosomal cap protein and a tail protein using monoclonal anti-mouse sperm antibodies. / Schmell, Eli D.; Gulyas, Bela J.; Yuan, Lydia C.; August, Thomas.

In: Journal of Reproductive Immunology, Vol. 4, No. 2, 1982, p. 91-106.

Research output: Contribution to journalArticle

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