A micro enzyme-linked immunosorbent assay (ELISA) for identifying malaria sporozoites in mosquitoes is described. Using an extract of dried infected mosquitoes as antigen, a two site ELISA was sensitive enough to detect one infected mosquito in a pool of 20. The species specificity, sensitivity and ease of performance of this assay, as well as the stability of the reagent, should make it a useful epidemiological tool.
ASJC Scopus subject areas
- Infectious Diseases