Identification of malaria-infected mosquitoes by a two-site enzyme-linked immunosorbent assay

T. R. Burkot, F. Zavala, R. W. Gwadz, F. H. Collins, R. S. Nussenzweig, D. R. Roberts

Research output: Contribution to journalArticle

Abstract

A micro enzyme-linked immunosorbent assay (ELISA) for identifying malaria sporozoites in mosquitoes is described. Using an extract of dried infected mosquitoes as antigen, a two site ELISA was sensitive enough to detect one infected mosquito in a pool of 20. The species specificity, sensitivity and ease of performance of this assay, as well as the stability of the reagent, should make it a useful epidemiological tool.

Original languageEnglish (US)
Pages (from-to)227-231
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume33
Issue number2
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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