TY - JOUR
T1 - Identification of ligands for olfactory receptors by functional expression of a receptor library
AU - Krautwurst, Dietmar
AU - Yau, King Wai
AU - Reed, Randall R.
N1 - Funding Information:
We thank P. Hargrave, S. Offermanns, H. Zhao, and S. Firestein for reagents. We are grateful to S. Walsh for help with the sequence analysis and to S. Munger and R. Barber for comments on the manuscript. D. K. is grateful for continuous support and understanding from U. Krautwurst. D. K. was supported by a fellowship from the Deutsche Forschungsgemeinschaft. Work in the laboratories of K.-W. Y. and R. R. R is supported by HHMI.
PY - 1998/12/23
Y1 - 1998/12/23
N2 - The recognition of odorants by olfactory receptors represents the first stage in odor discrimination. Here, we report the generation of an expression library containing a large and diverse repertoire of mouse olfactory receptor sequences in the transmembrane II-VII region. From this library, 80 chimeric receptors were tested against 26 odorants after transfection into HEK-293 cells. Three receptors were identified to respond to micromolecular concentrations of carvone, (-) citronellal, and limonene, respectively. We also found that the mouse 17 receptor, unlike the rat 17 receptor, prefers heptanal instead of octanal, as a result of a single valine-to-isoleucine substitution. This finding represents the beginning of a molecular understanding of odorant recognition. The identification, on a large scale, of cognate receptor-odorant interactions should provide insight into olfactory coding mechanisms.
AB - The recognition of odorants by olfactory receptors represents the first stage in odor discrimination. Here, we report the generation of an expression library containing a large and diverse repertoire of mouse olfactory receptor sequences in the transmembrane II-VII region. From this library, 80 chimeric receptors were tested against 26 odorants after transfection into HEK-293 cells. Three receptors were identified to respond to micromolecular concentrations of carvone, (-) citronellal, and limonene, respectively. We also found that the mouse 17 receptor, unlike the rat 17 receptor, prefers heptanal instead of octanal, as a result of a single valine-to-isoleucine substitution. This finding represents the beginning of a molecular understanding of odorant recognition. The identification, on a large scale, of cognate receptor-odorant interactions should provide insight into olfactory coding mechanisms.
UR - http://www.scopus.com/inward/record.url?scp=0032417641&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032417641&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)81716-X
DO - 10.1016/S0092-8674(00)81716-X
M3 - Article
C2 - 9875846
AN - SCOPUS:0032417641
SN - 0092-8674
VL - 95
SP - 917
EP - 926
JO - Cell
JF - Cell
IS - 7
ER -