Identification of immunodominant epitopes in envelope glycoprotein of human T Lymphotropic virus type II

Renu B. Lal, Donna L. Rudolph, Jonathan E. Kaplan, Brian Hjelle, Paul H. Levine, John E. Coligan, Raphael P. Viscidii

Research output: Contribution to journalArticle

Abstract

A series of synthetic peptides derived from the envelope glycoprotein of human T lymphotropic virus type II (HTLV-II) was used in an enzyme immunoassay to determine the immunodominant epitopes of envelope glycoprotein. Of the 11 synthetic peptides spanning the external glycoprotein of HTLV-II (gp52) and the 3 from the transmembrane protein (gp21), 3 peptides from gp52 (termed Env-2085-102. Env-20-209, and Env-20173-256) reacted with most of the polymerase chain reaction-confirmed HTLV-II specimens (83, 95, and 76%, respectively); all other peptides reacted minimally with these specimens. Env-20173-209 reacted with a greater percentage (91 to 100%) of specimens from different risk groups, including intravenous drug users (n = 30), North American Indians (n = 13), Guaymi Indians from Panama (n = 22), and routine U.S. blood donors (n = 34), when compared with Env-2085-102 (73 to 100%) or Env-203219-256 (68 to 83%). Furthermore, Env-2085-102 and Env-202172-209 had some reactivity (8-25%) with serafrom HTLV-I-infected individuals, whereas Env-20219-256 reacted with 58% of HTLV-I specimens. We conclude that peptides Env-2085-102 and Env-202173-209 represent the type-specific immunodominant epitopes of HTLV-II external glycoprotein.

Original languageEnglish (US)
Pages (from-to)274-279
Number of pages6
JournalVirology
Volume186
Issue number1
DOIs
StatePublished - Jan 1992

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ASJC Scopus subject areas

  • Virology

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