Identification of hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein I as a biomarker for pancreatic ductal adenocarcinoma by protein biochip technology

Christophe Rosty, Laurence Christa, Scott Kuzdzal, William M. Baldwin, Marianna L. Zahurak, Françoise Carnot, Daniel Wan-Yui Chan, Marcia Canto, Keith D. Lillemoe, John L Cameron, Charles J. Yeo, Ralph H Hruban, Michael S Goggins

Research output: Contribution to journalArticle

Abstract

New biomarkers of pancreatic adenocarcinoma are needed to improve the early detection of this deadly disease. We performed surface enhanced laser desorption ionization (SELDI) mass spectrometry using ProteinChip technology (Ciphergen Biosystems, Fremont, CA) to screen for tially expressed proteins in pancreatic juice. Pancreatic juice samples obtained from patients undergoing pancreatectomy for pancreatic adenocarcinoma were compared with juice samples from patients with other pancreatic diseases. We identified a peak ∼16,570 daltons present in the pancreatic juice from 10/15 (67%) of the patients with pancreatic adenocarcinoma and in the pancreatic juice from 1/7 (17%) of the patients with other pancreatic diseases. Using a ProteinChip immunoassay, we identified this differentially expressed protein as hepatocarcinoma-intestine-pancreas/pancreatitis-associated-protein I (HIP/PAP-I), a protein released from pancreatic acini during acute pancreatitis and overexpressed in hepatocellular carcinoma. We then quantified by ELISA the pancreatic juice HIP/PAP-I levels in 43 patients (28 with pancreatic adenocarcinoma, 15 with other pancreatic diseases) and the serum HIP/PAP-I levels in 98 patients (53 with pancreatic adenocarcinoma, 45 with other pancreatic diseases or healthy individuals). HIP/PAP-I levels were significantly higher in both the pancreatic juice (P <0.001) and in the serum (P <0.001) of patients with pancreatic adenocarcinoma compared with the control group. HIP/PAP-I levels were ∼-1000-fold higher in pancreatic juice compared with serum and the magnitude of the difference between the pancreatic adenocarcinoma group and the control group was greater in the pancreatic juice samples (143.75 ± 235.52 μg/ml versus 6.04 ±: 7.59 μg/ml) than in the serum samples (99.96 ± 140.66 ng/ml versus 35.25 ± 28.44 ng/ml). In our study, patients with pancreatic juice HIP/PAP-I levels ≥20 μg/ml were 21.9 times (95% confidence interval, 3.5-136.5; P <0.001) more likely to have pancreatic adenocarcinoma than patients with levels <20 μg/ml. Immunolabeling of tissue sections revealed that the HIP/PAP-I protein was strongly expressed in acini adjacent to the invasive adenocarcinoma, but it was only rarely (1/30; 3%) expressed in the neoplastic epithelium, which suggests that the main source of HIP/PAP-I release in the pancreatic juice is acini. This low level of HIP/PAP-I expression in pancreatic adenocarcinoma was confirmed by reverse transcription-PCR: only 1 (5%) of 19 pancreatic cancer cell lines expressed HIP/PAP-I transcripts. Taken together, these data suggest that pancreatic juice measurement of HIP/PAP-I may help to identify patients with pancreatic adenocarcinoma.

Original languageEnglish (US)
Pages (from-to)1868-1875
Number of pages8
JournalCancer Research
Volume62
Issue number6
StatePublished - Mar 15 2002

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Pancreatic Juice
Protein Array Analysis
Intestines
Pancreas
Adenocarcinoma
Biomarkers
Technology
Pancreatic Diseases
Serum
Proteins
pancreatitis-associated protein
Control Groups
Pancreatectomy
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Pancreatic Neoplasms
Immunoassay
Pancreatitis
Reverse Transcription
Early Diagnosis
Hepatocellular Carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Identification of hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein I as a biomarker for pancreatic ductal adenocarcinoma by protein biochip technology. / Rosty, Christophe; Christa, Laurence; Kuzdzal, Scott; Baldwin, William M.; Zahurak, Marianna L.; Carnot, Françoise; Chan, Daniel Wan-Yui; Canto, Marcia; Lillemoe, Keith D.; Cameron, John L; Yeo, Charles J.; Hruban, Ralph H; Goggins, Michael S.

In: Cancer Research, Vol. 62, No. 6, 15.03.2002, p. 1868-1875.

Research output: Contribution to journalArticle

Rosty, Christophe ; Christa, Laurence ; Kuzdzal, Scott ; Baldwin, William M. ; Zahurak, Marianna L. ; Carnot, Françoise ; Chan, Daniel Wan-Yui ; Canto, Marcia ; Lillemoe, Keith D. ; Cameron, John L ; Yeo, Charles J. ; Hruban, Ralph H ; Goggins, Michael S. / Identification of hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein I as a biomarker for pancreatic ductal adenocarcinoma by protein biochip technology. In: Cancer Research. 2002 ; Vol. 62, No. 6. pp. 1868-1875.
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abstract = "New biomarkers of pancreatic adenocarcinoma are needed to improve the early detection of this deadly disease. We performed surface enhanced laser desorption ionization (SELDI) mass spectrometry using ProteinChip technology (Ciphergen Biosystems, Fremont, CA) to screen for tially expressed proteins in pancreatic juice. Pancreatic juice samples obtained from patients undergoing pancreatectomy for pancreatic adenocarcinoma were compared with juice samples from patients with other pancreatic diseases. We identified a peak ∼16,570 daltons present in the pancreatic juice from 10/15 (67{\%}) of the patients with pancreatic adenocarcinoma and in the pancreatic juice from 1/7 (17{\%}) of the patients with other pancreatic diseases. Using a ProteinChip immunoassay, we identified this differentially expressed protein as hepatocarcinoma-intestine-pancreas/pancreatitis-associated-protein I (HIP/PAP-I), a protein released from pancreatic acini during acute pancreatitis and overexpressed in hepatocellular carcinoma. We then quantified by ELISA the pancreatic juice HIP/PAP-I levels in 43 patients (28 with pancreatic adenocarcinoma, 15 with other pancreatic diseases) and the serum HIP/PAP-I levels in 98 patients (53 with pancreatic adenocarcinoma, 45 with other pancreatic diseases or healthy individuals). HIP/PAP-I levels were significantly higher in both the pancreatic juice (P <0.001) and in the serum (P <0.001) of patients with pancreatic adenocarcinoma compared with the control group. HIP/PAP-I levels were ∼-1000-fold higher in pancreatic juice compared with serum and the magnitude of the difference between the pancreatic adenocarcinoma group and the control group was greater in the pancreatic juice samples (143.75 ± 235.52 μg/ml versus 6.04 ±: 7.59 μg/ml) than in the serum samples (99.96 ± 140.66 ng/ml versus 35.25 ± 28.44 ng/ml). In our study, patients with pancreatic juice HIP/PAP-I levels ≥20 μg/ml were 21.9 times (95{\%} confidence interval, 3.5-136.5; P <0.001) more likely to have pancreatic adenocarcinoma than patients with levels <20 μg/ml. Immunolabeling of tissue sections revealed that the HIP/PAP-I protein was strongly expressed in acini adjacent to the invasive adenocarcinoma, but it was only rarely (1/30; 3{\%}) expressed in the neoplastic epithelium, which suggests that the main source of HIP/PAP-I release in the pancreatic juice is acini. This low level of HIP/PAP-I expression in pancreatic adenocarcinoma was confirmed by reverse transcription-PCR: only 1 (5{\%}) of 19 pancreatic cancer cell lines expressed HIP/PAP-I transcripts. Taken together, these data suggest that pancreatic juice measurement of HIP/PAP-I may help to identify patients with pancreatic adenocarcinoma.",
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T1 - Identification of hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein I as a biomarker for pancreatic ductal adenocarcinoma by protein biochip technology

AU - Rosty, Christophe

AU - Christa, Laurence

AU - Kuzdzal, Scott

AU - Baldwin, William M.

AU - Zahurak, Marianna L.

AU - Carnot, Françoise

AU - Chan, Daniel Wan-Yui

AU - Canto, Marcia

AU - Lillemoe, Keith D.

AU - Cameron, John L

AU - Yeo, Charles J.

AU - Hruban, Ralph H

AU - Goggins, Michael S

PY - 2002/3/15

Y1 - 2002/3/15

N2 - New biomarkers of pancreatic adenocarcinoma are needed to improve the early detection of this deadly disease. We performed surface enhanced laser desorption ionization (SELDI) mass spectrometry using ProteinChip technology (Ciphergen Biosystems, Fremont, CA) to screen for tially expressed proteins in pancreatic juice. Pancreatic juice samples obtained from patients undergoing pancreatectomy for pancreatic adenocarcinoma were compared with juice samples from patients with other pancreatic diseases. We identified a peak ∼16,570 daltons present in the pancreatic juice from 10/15 (67%) of the patients with pancreatic adenocarcinoma and in the pancreatic juice from 1/7 (17%) of the patients with other pancreatic diseases. Using a ProteinChip immunoassay, we identified this differentially expressed protein as hepatocarcinoma-intestine-pancreas/pancreatitis-associated-protein I (HIP/PAP-I), a protein released from pancreatic acini during acute pancreatitis and overexpressed in hepatocellular carcinoma. We then quantified by ELISA the pancreatic juice HIP/PAP-I levels in 43 patients (28 with pancreatic adenocarcinoma, 15 with other pancreatic diseases) and the serum HIP/PAP-I levels in 98 patients (53 with pancreatic adenocarcinoma, 45 with other pancreatic diseases or healthy individuals). HIP/PAP-I levels were significantly higher in both the pancreatic juice (P <0.001) and in the serum (P <0.001) of patients with pancreatic adenocarcinoma compared with the control group. HIP/PAP-I levels were ∼-1000-fold higher in pancreatic juice compared with serum and the magnitude of the difference between the pancreatic adenocarcinoma group and the control group was greater in the pancreatic juice samples (143.75 ± 235.52 μg/ml versus 6.04 ±: 7.59 μg/ml) than in the serum samples (99.96 ± 140.66 ng/ml versus 35.25 ± 28.44 ng/ml). In our study, patients with pancreatic juice HIP/PAP-I levels ≥20 μg/ml were 21.9 times (95% confidence interval, 3.5-136.5; P <0.001) more likely to have pancreatic adenocarcinoma than patients with levels <20 μg/ml. Immunolabeling of tissue sections revealed that the HIP/PAP-I protein was strongly expressed in acini adjacent to the invasive adenocarcinoma, but it was only rarely (1/30; 3%) expressed in the neoplastic epithelium, which suggests that the main source of HIP/PAP-I release in the pancreatic juice is acini. This low level of HIP/PAP-I expression in pancreatic adenocarcinoma was confirmed by reverse transcription-PCR: only 1 (5%) of 19 pancreatic cancer cell lines expressed HIP/PAP-I transcripts. Taken together, these data suggest that pancreatic juice measurement of HIP/PAP-I may help to identify patients with pancreatic adenocarcinoma.

AB - New biomarkers of pancreatic adenocarcinoma are needed to improve the early detection of this deadly disease. We performed surface enhanced laser desorption ionization (SELDI) mass spectrometry using ProteinChip technology (Ciphergen Biosystems, Fremont, CA) to screen for tially expressed proteins in pancreatic juice. Pancreatic juice samples obtained from patients undergoing pancreatectomy for pancreatic adenocarcinoma were compared with juice samples from patients with other pancreatic diseases. We identified a peak ∼16,570 daltons present in the pancreatic juice from 10/15 (67%) of the patients with pancreatic adenocarcinoma and in the pancreatic juice from 1/7 (17%) of the patients with other pancreatic diseases. Using a ProteinChip immunoassay, we identified this differentially expressed protein as hepatocarcinoma-intestine-pancreas/pancreatitis-associated-protein I (HIP/PAP-I), a protein released from pancreatic acini during acute pancreatitis and overexpressed in hepatocellular carcinoma. We then quantified by ELISA the pancreatic juice HIP/PAP-I levels in 43 patients (28 with pancreatic adenocarcinoma, 15 with other pancreatic diseases) and the serum HIP/PAP-I levels in 98 patients (53 with pancreatic adenocarcinoma, 45 with other pancreatic diseases or healthy individuals). HIP/PAP-I levels were significantly higher in both the pancreatic juice (P <0.001) and in the serum (P <0.001) of patients with pancreatic adenocarcinoma compared with the control group. HIP/PAP-I levels were ∼-1000-fold higher in pancreatic juice compared with serum and the magnitude of the difference between the pancreatic adenocarcinoma group and the control group was greater in the pancreatic juice samples (143.75 ± 235.52 μg/ml versus 6.04 ±: 7.59 μg/ml) than in the serum samples (99.96 ± 140.66 ng/ml versus 35.25 ± 28.44 ng/ml). In our study, patients with pancreatic juice HIP/PAP-I levels ≥20 μg/ml were 21.9 times (95% confidence interval, 3.5-136.5; P <0.001) more likely to have pancreatic adenocarcinoma than patients with levels <20 μg/ml. Immunolabeling of tissue sections revealed that the HIP/PAP-I protein was strongly expressed in acini adjacent to the invasive adenocarcinoma, but it was only rarely (1/30; 3%) expressed in the neoplastic epithelium, which suggests that the main source of HIP/PAP-I release in the pancreatic juice is acini. This low level of HIP/PAP-I expression in pancreatic adenocarcinoma was confirmed by reverse transcription-PCR: only 1 (5%) of 19 pancreatic cancer cell lines expressed HIP/PAP-I transcripts. Taken together, these data suggest that pancreatic juice measurement of HIP/PAP-I may help to identify patients with pancreatic adenocarcinoma.

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