Identification of glutamate transporters and receptors in mouse testis

Jia Hua Hu, Na Yang, Ying Hua Ma, Jie Jiang, Jin Fu Zhang, Jian Fei, Li He Guo

Research output: Contribution to journalArticlepeer-review

Abstract

AIM: To investigate the presence of glutamate transporters and receptors in mouse testis. METHODS: Glutamate uptake analysis was performed to study the function of glutamate transporters in mouse testis. Comparative RT-PCR technique and sequencing analysis were used to study the expression of glutamate receptors and transporters in mouse testis. RESULTS: Mouse testis possessed glutamate uptake capacity with sodium-dependence. Vmax value of glutamate uptake was (1.60 ± 0.21) pmol/min per mg protein and Km value of glutamate uptake was (11.0±1.6) μmol/L in mouse testis according to saturation analysis. Furthermore, the uptake activity could be inhibited by DHK (GLT1 selective inhibitor) and THA (glutamate uptake inhibitor). In addition, RT-PCR results revealed that glutamate transporters (GLT1 and EAAC1) and ionotropic glutamate receptors (NR1, NR2B, GluR6 and KA2) were expressed in mouse testis. CONCLUSION: Glutamate transporters and receptors do exist in mouse testis.

Original languageEnglish (US)
Pages (from-to)366-371
Number of pages6
JournalActa Pharmacologica Sinica
Volume25
Issue number3
StatePublished - Mar 1 2004

Keywords

  • Amino acid transport systems
  • Glutamates
  • Receptors
  • Testis
  • Uptake

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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