Identification of globin mRNA in 10s RNA of rabbit reticulocytes

Haig H. Kazazian, Patricia A. Moore, Pamela G. Snyder

Research output: Contribution to journalArticle

Abstract

Electrophoresis on 6% polyacrylamide gels splits 10s RNA of detergenttreated polysomes from rabbit reticulocytes into two major bands. After these two RNAs are isolated separately, the first 10s RNA1 1 The 10s RNA with greater electrophoretic mobility toward the anode is designated "first 10s RNA." The slower 10s RNA is "second 10s RNA." directs the synthesis of both α and β chains in the Krebs II ascites cell-free system. In contrast, the second 10s RNA is inactive in directing globin synthesis. This result is further documented by separation of the two 10s RNAs by oligo dT-cellulose chromatography and by isolation of globin mRNA after EDTA-treatment of reticulocyte polysomes. Therefore, globin mRNA containing both α- and β-chain synthetic capacity moves as a single RNA species on electrophoresis in polyacrylamide gels.

Original languageEnglish (US)
Pages (from-to)564-571
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume51
Issue number3
DOIs
StatePublished - Apr 2 1973

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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