Identification of globin mRNA in 10s RNA of rabbit reticulocytes

Haig Kazazian, Patricia A. Moore, Pamela G. Snyder

Research output: Contribution to journalArticle

Abstract

Electrophoresis on 6% polyacrylamide gels splits 10s RNA of detergenttreated polysomes from rabbit reticulocytes into two major bands. After these two RNAs are isolated separately, the first 10s RNA1 1 The 10s RNA with greater electrophoretic mobility toward the anode is designated "first 10s RNA." The slower 10s RNA is "second 10s RNA." directs the synthesis of both α and β chains in the Krebs II ascites cell-free system. In contrast, the second 10s RNA is inactive in directing globin synthesis. This result is further documented by separation of the two 10s RNAs by oligo dT-cellulose chromatography and by isolation of globin mRNA after EDTA-treatment of reticulocyte polysomes. Therefore, globin mRNA containing both α- and β-chain synthetic capacity moves as a single RNA species on electrophoresis in polyacrylamide gels.

Original languageEnglish (US)
Pages (from-to)564-571
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume51
Issue number3
DOIs
StatePublished - Apr 2 1973

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Globins
Reticulocytes
RNA
Rabbits
Messenger RNA
Polyribosomes
Electrophoresis
Electrophoretic mobility
Cell-Free System
Chromatography
Ascites
Edetic Acid
Polyacrylamide Gel Electrophoresis
Anodes
Electrodes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of globin mRNA in 10s RNA of rabbit reticulocytes. / Kazazian, Haig; Moore, Patricia A.; Snyder, Pamela G.

In: Biochemical and Biophysical Research Communications, Vol. 51, No. 3, 02.04.1973, p. 564-571.

Research output: Contribution to journalArticle

Kazazian, Haig ; Moore, Patricia A. ; Snyder, Pamela G. / Identification of globin mRNA in 10s RNA of rabbit reticulocytes. In: Biochemical and Biophysical Research Communications. 1973 ; Vol. 51, No. 3. pp. 564-571.
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