Identification of genital tract papillomaviruses HPV-6 and HPV-16 in warts of the oral cavity

Z. Naghashfar, E. Sawada, M. J. Kutcher, J. Swancar, J. Gupta, R. Daniel, H. Kashima, J. D. Woodruff, K. Shah

Research output: Contribution to journalArticle

Abstract

Warty lesions of the oral cavity were examined for etiologic association with genital tract papillomaviruses HPV-6, HPV-11, and HPV-16. DNAs extracted from ten oral biopsies were screened for HPV genomic sequences by Southern transfer hybridization with 32P-labeled viral DNA probes. Nonstringent hybridization with an HPV-6 probe revealed papillomavirus DNA sequences in four of seven tissues with histologic evidence of papillomatosis, in none of two tissues without histologic evidence of papillomatosis, and in one tissue that was not examined by histology. Stringent hybridization tests with HPV-6 and HPV-16 probes identified the genome in one tissue as being HPV-16, in a second tissue as being HPV-6 subtype a, and in a third tissue as HPV-6 (subtype unidentified); papillomavirus DNA sequences in two tissues are as yet not identified. An additional case of HPV-6 or HPV-11 related oral cavity lesion was diagnosed by in situ hybridization of paraffin sections with a 35S-labeled, mixed HPV-6 + HPV-11 probe. The hybridization in the positive section was extensive and confined to epithelial nuclei. The oral lesions associated with genital tract papilloma-viruses were asymptomatic, multiple or single, and were located in different parts of the oral cavity, for example, on the gingivae, on the tongue, on the lip, on the tonsillar pillar, and on the floor of the mouth.

Original languageEnglish (US)
Pages (from-to)313-324
Number of pages12
JournalJournal of Medical Virology
Volume17
Issue number4
DOIs
StatePublished - 1985

Fingerprint

Human papillomavirus 6
Warts
Human papillomavirus 16
Mouth
Human papillomavirus 11
Papilloma
Mouth Floor
DNA Probes
Viral DNA
Gingiva
Lip
Tongue
Paraffin
In Situ Hybridization
Histology
Genome
Viruses
Biopsy
DNA

ASJC Scopus subject areas

  • Virology

Cite this

Naghashfar, Z., Sawada, E., Kutcher, M. J., Swancar, J., Gupta, J., Daniel, R., ... Shah, K. (1985). Identification of genital tract papillomaviruses HPV-6 and HPV-16 in warts of the oral cavity. Journal of Medical Virology, 17(4), 313-324. https://doi.org/10.1002/jmv.1890170404

Identification of genital tract papillomaviruses HPV-6 and HPV-16 in warts of the oral cavity. / Naghashfar, Z.; Sawada, E.; Kutcher, M. J.; Swancar, J.; Gupta, J.; Daniel, R.; Kashima, H.; Woodruff, J. D.; Shah, K.

In: Journal of Medical Virology, Vol. 17, No. 4, 1985, p. 313-324.

Research output: Contribution to journalArticle

Naghashfar, Z, Sawada, E, Kutcher, MJ, Swancar, J, Gupta, J, Daniel, R, Kashima, H, Woodruff, JD & Shah, K 1985, 'Identification of genital tract papillomaviruses HPV-6 and HPV-16 in warts of the oral cavity', Journal of Medical Virology, vol. 17, no. 4, pp. 313-324. https://doi.org/10.1002/jmv.1890170404
Naghashfar, Z. ; Sawada, E. ; Kutcher, M. J. ; Swancar, J. ; Gupta, J. ; Daniel, R. ; Kashima, H. ; Woodruff, J. D. ; Shah, K. / Identification of genital tract papillomaviruses HPV-6 and HPV-16 in warts of the oral cavity. In: Journal of Medical Virology. 1985 ; Vol. 17, No. 4. pp. 313-324.
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