Identification of fibroblast growth factor-5 as an overexpressed antigen in multiple human adenocarcinomas

K. I. Hanada, D. M. Perry-Lalley, G. A. Ohnmacht, Maria Bettinotti, J. C. Yang

Research output: Contribution to journalArticle

Abstract

Methodology for identifying tumor-associated antigens recognized by T cells has been successfully used to clone antigens from melanoma cells. Similar efforts for nonmelanoma tumors have had limited success with few antigens identified. To identify potentially relevant tumor-associated antigens expressed in renal cell carcinoma cell lines, a tumor-specific CTL clone was established from tumor-infiltrating lymphocytes from a regressing pulmonary lesion. This CTL recognized nonmutated fibroblast growth factor-5 (FGF-5). Quantitative real-time reverse transcription PCR revealed that FGF-5 was overexpressed in the majority of renal cell carcinomas, as well as in some prostate carcinoma and breast carcinoma lines. FGF-5 expression by quantitative real-time reverse transcription PCR in normal tissues was below the recognition threshold for this CTL. As a normal protein with significant overexpression by multiple adenocarcinomas and little normal tissue expression, FGF-5 represents an immunotherapy target with potential utility against a broad array of nonmelanoma cancers.

Original languageEnglish (US)
Pages (from-to)5511-5516
Number of pages6
JournalCancer Research
Volume61
Issue number14
StatePublished - Jul 15 2001
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 5
Adenocarcinoma
Antigens
Neoplasm Antigens
Renal Cell Carcinoma
Reverse Transcription
Clone Cells
Melanoma-Specific Antigens
Tumor-Infiltrating Lymphocytes
Polymerase Chain Reaction
Tumor Cell Line
Immunotherapy
Prostate
Neoplasms
Breast Neoplasms
Carcinoma
T-Lymphocytes
Lung
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Hanada, K. I., Perry-Lalley, D. M., Ohnmacht, G. A., Bettinotti, M., & Yang, J. C. (2001). Identification of fibroblast growth factor-5 as an overexpressed antigen in multiple human adenocarcinomas. Cancer Research, 61(14), 5511-5516.

Identification of fibroblast growth factor-5 as an overexpressed antigen in multiple human adenocarcinomas. / Hanada, K. I.; Perry-Lalley, D. M.; Ohnmacht, G. A.; Bettinotti, Maria; Yang, J. C.

In: Cancer Research, Vol. 61, No. 14, 15.07.2001, p. 5511-5516.

Research output: Contribution to journalArticle

Hanada, KI, Perry-Lalley, DM, Ohnmacht, GA, Bettinotti, M & Yang, JC 2001, 'Identification of fibroblast growth factor-5 as an overexpressed antigen in multiple human adenocarcinomas', Cancer Research, vol. 61, no. 14, pp. 5511-5516.
Hanada, K. I. ; Perry-Lalley, D. M. ; Ohnmacht, G. A. ; Bettinotti, Maria ; Yang, J. C. / Identification of fibroblast growth factor-5 as an overexpressed antigen in multiple human adenocarcinomas. In: Cancer Research. 2001 ; Vol. 61, No. 14. pp. 5511-5516.
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