Identification of factor XIII-A as a marker of alternative macrophage activation

D. Törocsik, H. Bárdos, L. Nagy, R. Ádány

Research output: Contribution to journalArticle

Abstract

Factor XIII subunit A of blood coagulation (FXIII-A) is known to be synthesized but not secreted by the monocyte/macrophage cell line. On the basis of its intracellular localization and substrate profile, FXIII-A is thought to be involved in certain intracellular processes. Our present study was designed to monitor the changes in FXIII-A gene expression and protein production in long-term culture of human monocytes during their differentiation into macrophages in the presence of activating agents (interleukin-4, interferon-γ, Mycobacterium bovis BCG) inducing classical and alternative activation pathways. By using quantitative RT-PCR and fluorescent image analysis at the single-cell level we demonstrated that the expression of FXIII-A both at the mRNA as well as at the protein level is inversely regulated during the two activation programmes. Here we conclude that FXIII-A expression is an intracellular marker for alternatively activated macrophages, while its absence in monocyte-derived macrophages indicates their classically activated state.

Original languageEnglish (US)
Pages (from-to)2132-2139
Number of pages8
JournalCellular and Molecular Life Sciences
Volume62
Issue number18
DOIs
StatePublished - Sep 1 2005
Externally publishedYes

    Fingerprint

Keywords

  • Alternative activation
  • Classical activation
  • Factor XIII-A
  • Gene expression
  • Macrophage

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this