Identification of essential components of the S. cerevisiae kinetochore

Kimberly Floy Doheny, Peter K. Sorger, Anthony A. Hyman, Stuart Tugendreich, Forrest Spencer, Philip Hieter

Research output: Contribution to journalArticlepeer-review

Abstract

We have designed and utilized two in vivo assays of kinetochore integrity in S. cerevisiae. One assay detects relaxation of a transcription block formed at centromeres; the other detects an increase in the mitotic stability of a dicentric test chromosome. ctf13-30 and ctf14-42 were identified as putative kinetochore mutants by both assays. CTF14 is identical to NDC10 CBF2, a recently identified essential gene that encodes a 110 kd kinetochore component. CTF13 is an essential gene that encodes a predicted 478 amino acid protein with no homology to known proteins. ctf13 mutants missegregate chromosomes at permissive temperature and transiently arrest at nonpermissive temperature as large-budded cells with a G2 DNA content and a short spindle. Antibodies recognizing epitope-tagged CTF13 protein decrease the electrophoretic mobility of a CEN DNA-protein complex formed in vitro. Together, the genetic and biochemical data indicate that CTF13 is an essential kinetochore protein.

Original languageEnglish (US)
Pages (from-to)761-774
Number of pages14
JournalCell
Volume73
Issue number4
DOIs
StatePublished - May 21 1993

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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