@article{b1b783f29a544425808e80846708a6d8,
title = "Identification of essential components of the S. cerevisiae kinetochore",
abstract = "We have designed and utilized two in vivo assays of kinetochore integrity in S. cerevisiae. One assay detects relaxation of a transcription block formed at centromeres; the other detects an increase in the mitotic stability of a dicentric test chromosome. ctf13-30 and ctf14-42 were identified as putative kinetochore mutants by both assays. CTF14 is identical to NDC10 CBF2, a recently identified essential gene that encodes a 110 kd kinetochore component. CTF13 is an essential gene that encodes a predicted 478 amino acid protein with no homology to known proteins. ctf13 mutants missegregate chromosomes at permissive temperature and transiently arrest at nonpermissive temperature as large-budded cells with a G2 DNA content and a short spindle. Antibodies recognizing epitope-tagged CTF13 protein decrease the electrophoretic mobility of a CEN DNA-protein complex formed in vitro. Together, the genetic and biochemical data indicate that CTF13 is an essential kinetochore protein.",
author = "Doheny, {Kimberly Floy} and Sorger, {Peter K.} and Hyman, {Anthony A.} and Stuart Tugendreich and Forrest Spencer and Philip Hieter",
note = "Funding Information: We thank C. Connelly for significant contributions to this work. We would like to thank Ft. Parker for sending pGAB, J. Kroll for allowing us to use p414GEU1, and A. Pluta for kindly providing us with poly clonal El antibodies. We would like to acknowledge S. Holloway, R. Sikorski, and N. Kouprina for helpful theoretical discussions and H. Varmus and T. Mitch&on for support during this project. We are also grateful to J. Kilmartin, J. Carbon, and J. Lechner for communicating results prior to publication. We thank D. Koshland, W. Earnshaw, and T. Kelly for critical reading of the manuscript. K. F. D. is a student in the predoctoral training program in human genetics at Johns Hopkins (National Institute of General Medical Sciences grant P32GM07614). S. T. is supported by the National Institutes of Health Departmental Training Grant 5T32CA09139. P. K. S. and A. A. H. are biomedical scholars of the Lucille P. Markey Charitable Trust. This work was supported by a National Institutes of Health grant (CA16519) to P. H. and an American Cancer Society grant (CD-509) to F. S.",
year = "1993",
month = may,
day = "21",
doi = "10.1016/0092-8674(93)90255-O",
language = "English (US)",
volume = "73",
pages = "761--774",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",
}