Identification of enteric pathogens in the small and large intestine of children with diarrhea

Peter Echeverria, David N. Taylor, Udom Leksomboon, Neil R. Blacklow, Sajee Pinnoi, James P. Nataro, James Kaper, Bernard Rowe

Research output: Contribution to journalArticlepeer-review

Abstract

Enteric pathogens were identified in children with diarrhea from duodenal specimens obtained with a string capsule and from fecal specimens. Rotavirus was identified in stools of 43 of 100 children, and was recovered from the small intestine from nine (21%) children who were excreting this virus. Shigella was isolated from stools from 22, Salmonella from 17, enterotoxigenic Escherichiacoli from eight, and Aeromonashydrophila from one of 100 children with diarrhea. In contrast to rotavirus, Salmonella, Shigella, enterotoxigenic Escherichiacoli, and A. hydrophila were not isolated from the small intestine. Nonenterotoxigenic Aeromonas species were recovered from the small intestine, but not the stool of five children. These children were also infected with Shigella or with rotavirus; this suggests that Aeromonas was not the cause of their diarrhea. None of 51 Escherichiacoli isolated with the string capsules, or 67 isolated from stool that agglutinated in commercial enteropathogenic Escherichiacoli antisera were of classical enteropathogenic Escherichiacoli serotypes. One hundred and five of these 118 Escherichiacoli did not hybridize with a deoxyribonucleic acid probe for plasmid mediated factors conferring adherence to HeLa cells. Examination of specimens collected with a string capsule from children with diarrhea did not identify any more enteric pathogens than examining stools. Furthermore testing Escherichiacoli for agglutination in commercial enteropathogenic Escherichiacoli antisera did not identify Escherichiacoli of enteropathogenic serotypes.

Original languageEnglish (US)
Pages (from-to)277-284
Number of pages8
JournalDiagnostic Microbiology and Infectious Disease
Volume4
Issue number4
DOIs
StatePublished - Apr 1986

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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