Identification of Ehrlichia chaffeensis by nested PCR in ticks from southern China

Wu Chun Cao, Yu Min Gao, Pan He Zhang, Xi Tan Zhang, Qing Hua Dai, J. Stephen Dumler, Li Qun Fang, Hong Yang

Research output: Contribution to journalArticle

Abstract

A total of 717 ticks collected from southern China were examined by nested PCR for the presence of Ehrlichia chaffeensis. Sixteen (55.2%) of 29 adult Amblyomma testudinarium ticks and 28 (11.7%) of 240 adult and at least 4.2% of 215 nymphal (pooled specimens) Haemaphysalis yeni ticks tested positive. Four other species of ticks were negative. Selected positive amplicons were confirmed by DNA sequencing.

Original languageEnglish (US)
Pages (from-to)2778-2780
Number of pages3
JournalJournal of clinical microbiology
Volume38
Issue number7
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Microbiology (medical)

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    Cao, W. C., Gao, Y. M., Zhang, P. H., Zhang, X. T., Dai, Q. H., Dumler, J. S., Fang, L. Q., & Yang, H. (2000). Identification of Ehrlichia chaffeensis by nested PCR in ticks from southern China. Journal of clinical microbiology, 38(7), 2778-2780. https://doi.org/10.1128/jcm.38.7.2778-2780.2000