Identification of EGF as an angiogenic factor present in conditioned medium from human salivary gland adenocarcinoma cell clones with varying degrees of metastatic potential

Masayuki Azuma, Tetsuya Tamatani, Kazuhiro Fukui, Tokuyuki Yuki, Hideo Yoshida, Takashi Bando, Mohammad Hoque, Takashi Kamogashira, Koichi Ogino, Naoki Nishino, Toshimitsu Suzuki, Mitsunobu Sato

Research output: Contribution to journalArticle

Abstract

We have previously shown that conditioned medium (CM) from metastasizing human salivary gland adenocarcinoma cell clones contains factor(s) that stimulate the proliferation and migration of bovine aortic endothelial (BAE) cells, and inhibit the production of collagenases by BAE cells (Azuma M. et al. (1993) Cancer Lett., 73, 85-93). To further characterize this, we evaluated the expression level of epidermal growth factor (EGF) secreted by a non-metastasizing cell clone (HSGc) and its metastasizing cell clones, and analysed the effect of EGF on the biologic behaviors of BAE cells. When the secretion of EGF by cell clones was estimated by enzyme-linked immunosorbent assay, metastasizing cell clones released a large amount of EGF as compared with HSGc. However, the number of EGF receptor was detected consistently at a level that was similar in all cell clones. With regard to the effect of EGF on the malignant potential of cell clones such as proteolytic aggressiveness, EGF did not affect the secretion of both collagenases and their inhibitor from cell clones. Alternatively, exogenous EGF stimulated the proliferation and migration of BAE cells, and inhibited the secretion of collagenases from BAE cells. Neutralization with a neutralizing antibody of EGF released into CM abolished the inhibitory effect of CM on the secretion of collagenases from BAE cells. Thus, the CM-contained factor, which is responsible for the induction of biologic behaviors of BAE cells, can be attributed to EGF.

Original languageEnglish (US)
Pages (from-to)189-198
Number of pages10
JournalCancer Letters
Volume84
Issue number2
DOIs
StatePublished - Sep 15 1994
Externally publishedYes

Fingerprint

Angiogenesis Inducing Agents
Conditioned Culture Medium
Salivary Glands
Epidermal Growth Factor
Adenocarcinoma
Clone Cells
Endothelial Cells
Collagenases
Matrix Metalloproteinase Inhibitors
Neutralizing Antibodies
Epidermal Growth Factor Receptor
Enzyme-Linked Immunosorbent Assay

Keywords

  • Angiogenesis
  • EGF
  • Human salivary gland adenocarcinoma cells
  • Metastasis

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Identification of EGF as an angiogenic factor present in conditioned medium from human salivary gland adenocarcinoma cell clones with varying degrees of metastatic potential. / Azuma, Masayuki; Tamatani, Tetsuya; Fukui, Kazuhiro; Yuki, Tokuyuki; Yoshida, Hideo; Bando, Takashi; Hoque, Mohammad; Kamogashira, Takashi; Ogino, Koichi; Nishino, Naoki; Suzuki, Toshimitsu; Sato, Mitsunobu.

In: Cancer Letters, Vol. 84, No. 2, 15.09.1994, p. 189-198.

Research output: Contribution to journalArticle

Azuma, M, Tamatani, T, Fukui, K, Yuki, T, Yoshida, H, Bando, T, Hoque, M, Kamogashira, T, Ogino, K, Nishino, N, Suzuki, T & Sato, M 1994, 'Identification of EGF as an angiogenic factor present in conditioned medium from human salivary gland adenocarcinoma cell clones with varying degrees of metastatic potential', Cancer Letters, vol. 84, no. 2, pp. 189-198. https://doi.org/10.1016/0304-3835(94)90375-1
Azuma, Masayuki ; Tamatani, Tetsuya ; Fukui, Kazuhiro ; Yuki, Tokuyuki ; Yoshida, Hideo ; Bando, Takashi ; Hoque, Mohammad ; Kamogashira, Takashi ; Ogino, Koichi ; Nishino, Naoki ; Suzuki, Toshimitsu ; Sato, Mitsunobu. / Identification of EGF as an angiogenic factor present in conditioned medium from human salivary gland adenocarcinoma cell clones with varying degrees of metastatic potential. In: Cancer Letters. 1994 ; Vol. 84, No. 2. pp. 189-198.
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AU - Yuki, Tokuyuki

AU - Yoshida, Hideo

AU - Bando, Takashi

AU - Hoque, Mohammad

AU - Kamogashira, Takashi

AU - Ogino, Koichi

AU - Nishino, Naoki

AU - Suzuki, Toshimitsu

AU - Sato, Mitsunobu

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