Identification of cryptic sites of DNA sequence amplification in human breast cancer by chromosome microdissection

Xin Yuan Guan; Paul S. Meltzer; William S. Dalton; Jeffrey M. Trent

doi:10.1038/ng1094-155

Identification of cryptic sites of DNA sequence amplification in human breast cancer by chromosome microdissection

Xin Yuan Guan, Paul S. Meltzer, William S. Dalton, Jeffrey M. Trent

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139 Scopus citations

Abstract

We have performed microdissection of 16 putative homogeneously staining regions (hsrs) from nine different breast cancer cell lines in order to determine their chromosomal origin and composition. As expected, the most commonly amplified chromosomal band-region was 17q12 (containing ERBB2). However, regions not containing known oncogenes were also identified, including 13q31 (5/9 cases) and 20q12–13.2 (4/9 cases). The chromosomal composition of the integrated amplified DNA within each hsr was determined and in 13/16 cases (81 %), hsrs were shown to be composed of two or more chromosomal regions. These studies shed light on the mechanism of formation of hsrs, and identify chromosomal regions likely to harbour genes amplified in breast cancer.

Original language	English (US)
Pages (from-to)	155-161
Number of pages	7
Journal	Nature genetics
Volume	8
Issue number	2
DOIs	https://doi.org/10.1038/ng1094-155
State	Published - Oct 1994
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "Identification of cryptic sites of DNA sequence amplification in human breast cancer by chromosome microdissection",

abstract = "We have performed microdissection of 16 putative homogeneously staining regions (hsrs) from nine different breast cancer cell lines in order to determine their chromosomal origin and composition. As expected, the most commonly amplified chromosomal band-region was 17q12 (containing ERBB2). However, regions not containing known oncogenes were also identified, including 13q31 (5/9 cases) and 20q12–13.2 (4/9 cases). The chromosomal composition of the integrated amplified DNA within each hsr was determined and in 13/16 cases (81 %), hsrs were shown to be composed of two or more chromosomal regions. These studies shed light on the mechanism of formation of hsrs, and identify chromosomal regions likely to harbour genes amplified in breast cancer.",

author = "Guan, {Xin Yuan} and Meltzer, {Paul S.} and Dalton, {William S.} and Trent, {Jeffrey M.}",

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AU - Guan, Xin Yuan

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AU - Trent, Jeffrey M.

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AB - We have performed microdissection of 16 putative homogeneously staining regions (hsrs) from nine different breast cancer cell lines in order to determine their chromosomal origin and composition. As expected, the most commonly amplified chromosomal band-region was 17q12 (containing ERBB2). However, regions not containing known oncogenes were also identified, including 13q31 (5/9 cases) and 20q12–13.2 (4/9 cases). The chromosomal composition of the integrated amplified DNA within each hsr was determined and in 13/16 cases (81 %), hsrs were shown to be composed of two or more chromosomal regions. These studies shed light on the mechanism of formation of hsrs, and identify chromosomal regions likely to harbour genes amplified in breast cancer.

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Identification of cryptic sites of DNA sequence amplification in human breast cancer by chromosome microdissection

Abstract

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