Identification of Candida albicans and Candida glabrata within 1.5 hours directly from positive blood culture bottles with a shortened peptide nucleic acid fluorescence in situ hybridization protocol

Michael Gherna, William G. Merz

Research output: Contribution to journalArticle

Abstract

Candida albicans and Candida glabrata can be identified in blood culture bottles within 2.5 h using peptide nucleic acid fluorescence in situ hybridization. A 1.25-h protocol was compared to the standard with 40 positive (clinical and spiked) blood culture bottles tested in batches of 5. All C. albicans (15) and C. glabrata (16) isolates, alone or mixed, were identified correctly using both protocols, whereas 18 isolates (five other species) were negative by both protocols. This shortened method will significantly reduce the time to identification.

Original languageEnglish (US)
Pages (from-to)247-248
Number of pages2
JournalJournal of Clinical Microbiology
Volume47
Issue number1
DOIs
StatePublished - Jan 2009

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Peptide Nucleic Acids
Candida glabrata
Candida albicans
Fluorescence In Situ Hybridization
Blood Culture

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

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