Identification of c-Myc responsive genes using rat cDNA microarray

Q. M. Guo, R. L. Malek, S. Kim, C. Chiao, M. He, M. Ruffy, K. Sanka, N. H. Lee, C. V. Dang, E. T. Liu

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Abstract

c-Myc functions through direct activation or repression of transcription. Using cDNA microarray analysis, we have identified c-Myc-responsive genes by comparing gene expression profiles between c-myc null and c-myc wild-type rat fibroblast cells and between c-myc null and c-myc null cells reconstituted with c-myc. From a panel of 4400 cDNA elements, we found 198 genes responsive to c-myc when comparing wild-type or reconstituted cells with the null cells. The plurality of the named c-Myc-responsive genes that were up-regulated, including 30 ribosomal protein genes, are involved in macro-molecular synthesis and metabolism, suggesting a major role of c-Myc in the regulation of protein synthetic and metabolic pathways. When ectopically overexpressed, c-Myc induced a different and smaller set of c-Myc-responsive genes as compared with the physiologically expressed c-Myc condition. Thus, these results from expression profiling suggest a new primary function for c-Myc and raise the possibility that the physiological and transforming functions of c-myc may be separable.

Original languageEnglish (US)
Pages (from-to)5922-5928
Number of pages7
JournalCancer Research
Volume60
Issue number21
StatePublished - Nov 1 2000

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Guo, Q. M., Malek, R. L., Kim, S., Chiao, C., He, M., Ruffy, M., Sanka, K., Lee, N. H., Dang, C. V., & Liu, E. T. (2000). Identification of c-Myc responsive genes using rat cDNA microarray. Cancer Research, 60(21), 5922-5928.