TY - JOUR
T1 - Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis
AU - Mueller, Markus
AU - Bunk, Sebastian
AU - Diterich, Isabel
AU - Weichel, Michael
AU - Rauter, Carolin
AU - Hassler, Dieter
AU - Hermann, Corinna
AU - Crameri, Reto
AU - Hartung, Thomas
PY - 2006/10
Y1 - 2006/10
N2 - The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.
AB - The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.
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U2 - 10.1128/JCM.00371-06
DO - 10.1128/JCM.00371-06
M3 - Article
C2 - 17021109
AN - SCOPUS:34248664053
VL - 44
SP - 3778
EP - 3780
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 10
ER -