Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis

Markus Mueller, Sebastian Bunk, Isabel Diterich, Michael Weichel, Carolin Rauter, Dieter Hassler, Corinna Hermann, Reto Crameri, Thomas Hartung

Research output: Contribution to journalArticlepeer-review

Abstract

The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.

Original languageEnglish (US)
Pages (from-to)3778-3780
Number of pages3
JournalJournal of clinical microbiology
Volume44
Issue number10
DOIs
StatePublished - Oct 2006
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)

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