Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis

Markus Mueller; Sebastian Bunk; Isabel Diterich; Michael Weichel; Carolin Rauter; Dieter Hassler; Corinna Hermann; Reto Crameri; Thomas Hartung

doi:10.1128/JCM.00371-06

Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis

Markus Mueller, Sebastian Bunk, Isabel Diterich, Michael Weichel, Carolin Rauter, Dieter Hassler, Corinna Hermann, Reto Crameri, Thomas Hartung

Research output: Contribution to journal › Article › peer-review

Abstract

The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.

Original language	English (US)
Pages (from-to)	3778-3780
Number of pages	3
Journal	Journal of clinical microbiology
Volume	44
Issue number	10
DOIs	https://doi.org/10.1128/JCM.00371-06
State	Published - Oct 2006
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.00371-06

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Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis. / Mueller, Markus; Bunk, Sebastian; Diterich, Isabel; Weichel, Michael; Rauter, Carolin; Hassler, Dieter; Hermann, Corinna; Crameri, Reto; Hartung, Thomas.

In: Journal of clinical microbiology, Vol. 44, No. 10, 10.2006, p. 3778-3780.

Research output: Contribution to journal › Article › peer-review

Mueller, Markus ; Bunk, Sebastian ; Diterich, Isabel ; Weichel, Michael ; Rauter, Carolin ; Hassler, Dieter ; Hermann, Corinna ; Crameri, Reto ; Hartung, Thomas. / Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis. In: Journal of clinical microbiology. 2006 ; Vol. 44, No. 10. pp. 3778-3780.

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abstract = "The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.",

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AU - Weichel, Michael

AU - Rauter, Carolin

AU - Hassler, Dieter

AU - Hermann, Corinna

AU - Crameri, Reto

AU - Hartung, Thomas

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AB - The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.

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Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis

Abstract

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