Identification of B cell epitopes recognized by antibodies specific for the tumor antigen NY-ESO-1 in cancer patients with spontaneous immune responses

Danila Valmori, Naira E. Souleimanian, Charles S. Hesdorffer, Gerd Ritter, Lloyd J. Old, Maha Ayyoub

Research output: Contribution to journalArticle

Abstract

Expression of the germ line antigen NY-ESO-1 in adult somatic tissues other than testis is strictly found in association with cancer. Patients bearing NY-ESO-1 expressing tumors often develop integrated specific immune responses to the antigen, encompassing T cell and antibody responses. Hence, detection of NY-ESO-1 specific antibody responses can be considered as a cancer biomarker of great interest. Here, we used synthetic peptides spanning the sequence of the NY-ESO-1 protein to assess antibody responses in cancer patients. This approach allowed the identification of peptides containing linear B cell epitopes. Some peptides were recognized by the majority of seropositive patients thus identifying several distinct regions of the protein containing frequently recognized B cell epitopes. The results of this study provide the first appraisal of the diversity of naturally-occurring NY-ESO-1 specific antibodies and could be instrumental in the monitoring of therapy-induced antibody responses in cancer patients receiving NY-ESO-1-based vaccines.

Original languageEnglish (US)
Pages (from-to)24-30
Number of pages7
JournalClinical Immunology
Volume117
Issue number1
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

B-Lymphocyte Epitopes
Neoplasm Antigens
Antibody Formation
Antibodies
Peptides
Neoplasms
Histocompatibility Antigens Class II
Tumor Biomarkers
Germ Cells
Testis
Proteins
Vaccines
T-Lymphocytes
Antigens

Keywords

  • Antibodies
  • B cell epitopes
  • Human
  • Peptides
  • Tumor immunity

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Identification of B cell epitopes recognized by antibodies specific for the tumor antigen NY-ESO-1 in cancer patients with spontaneous immune responses. / Valmori, Danila; Souleimanian, Naira E.; Hesdorffer, Charles S.; Ritter, Gerd; Old, Lloyd J.; Ayyoub, Maha.

In: Clinical Immunology, Vol. 117, No. 1, 10.2005, p. 24-30.

Research output: Contribution to journalArticle

Valmori, Danila ; Souleimanian, Naira E. ; Hesdorffer, Charles S. ; Ritter, Gerd ; Old, Lloyd J. ; Ayyoub, Maha. / Identification of B cell epitopes recognized by antibodies specific for the tumor antigen NY-ESO-1 in cancer patients with spontaneous immune responses. In: Clinical Immunology. 2005 ; Vol. 117, No. 1. pp. 24-30.
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