Identification of autonomously replicating sequence (ARS) elements in eukaryotic cells

Rosemary K. Clyne, Thomas J. Kelly

Research output: Contribution to journalArticlepeer-review

Abstract

Autonomously replicating sequence (ARS) elements were first identified in the budding yeast Saccharomyces cerevisiae as chromosomal DNA fragments that promoted high frequency of transformation and extrachromosomal maintenance of plasmid DNA. These specific sequence elements were subsequently shown to function as origins of DNA replication. Detailed analysis of the structure and function of ARS elements has been limited largely to S. cerevisiae and more recently the fission yeast Schizosaccharomyces pombe. Characterization of ARS activity in other eukaryotes is far less complete. Here we describe the ARS assay developed in yeast and its application to the study of origin function in other eukaryotes. Other available methods for detecting autonomous replication in these systems are also presented.

Original languageEnglish (US)
Pages (from-to)221-233
Number of pages13
JournalMethods: A Companion to Methods in Enzymology
Volume13
Issue number3
DOIs
StatePublished - Nov 1997

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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