TY - JOUR
T1 - Identification of anti-persister activity against uropathogenic escherichia coli from a clinical drug library
AU - Niu, Hongxia
AU - Cui, Peng
AU - Shi, Wanliang
AU - Zhang, Shuo
AU - Feng, Jie
AU - Wang, Yong
AU - Sullivan, David
AU - Zhang, Wenhong
AU - Zhu, Bingdong
AU - Zhang, Ying
N1 - Publisher Copyright:
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
PY - 2015/5/12
Y1 - 2015/5/12
N2 - Uropathogenic E. coli is a major cause of urinary tract infections (UTIs), but current antibiotics do not always effectively clear the persistent infection. To identify drugs that eliminate uropathogenic E. coli persisters, we screened a clinical drug library consisting of 1524 compounds using high throughput drug exposure assay in 96-well plates. Bacterial survival was assessed by growth on LB plates. We identified 14 drug candidates (tosufloxacin, colistin, sparfloxacin, moxifloxacin and gatifloxacin, enrofloxacin and sarafloxacin, octodrine, clofoctol, dibekacin, cephalosporin C, pazufloxacin, streptomycin and neomycin), which had high anti-persister activity. Among them, tosufloxacin and colistin had the highest anti-persister activity and could completely eradicate E. coli persisters in 3 days in vitro while the current UTI antibiotics failed to do so. Our findings may have implications for the development of a more effective treatment for UTIs.
AB - Uropathogenic E. coli is a major cause of urinary tract infections (UTIs), but current antibiotics do not always effectively clear the persistent infection. To identify drugs that eliminate uropathogenic E. coli persisters, we screened a clinical drug library consisting of 1524 compounds using high throughput drug exposure assay in 96-well plates. Bacterial survival was assessed by growth on LB plates. We identified 14 drug candidates (tosufloxacin, colistin, sparfloxacin, moxifloxacin and gatifloxacin, enrofloxacin and sarafloxacin, octodrine, clofoctol, dibekacin, cephalosporin C, pazufloxacin, streptomycin and neomycin), which had high anti-persister activity. Among them, tosufloxacin and colistin had the highest anti-persister activity and could completely eradicate E. coli persisters in 3 days in vitro while the current UTI antibiotics failed to do so. Our findings may have implications for the development of a more effective treatment for UTIs.
KW - Anti-persister activity
KW - Clinical drug library
KW - Escherichia coli
KW - Persisters
KW - Urinary tract infection
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U2 - 10.3390/antibiotics4020179
DO - 10.3390/antibiotics4020179
M3 - Article
C2 - 27025620
AN - SCOPUS:84994529832
SN - 2079-6382
VL - 4
SP - 179
EP - 187
JO - Antibiotics
JF - Antibiotics
IS - 2
ER -