Identification of a transposon Tn7-dependent DNA-binding activity that recognizes the ends of Tn7.

R. L. McKown, C. S. Waddell, L. K. Arciszewska, N. L. Craig

Research output: Contribution to journalArticle

Abstract

The bacterial transposon Tn7 is distinguished by its capacity for high-frequency transposition to a specific site in the Escherichia coli chromosome. tnsB is one of the five Tn7-encoded transposition genes. We have identified in vitro a tnsB-dependent DNA binding activity that interacts specifically with cis-acting transposition sequences at the Tn7 termini. Although the left and right termini of Tn7 are structurally distinct, each end contains several copies of a closely homologous 22-base-pair sequence. We present results indicating that this 22-base-pair repeat sequence is recognized by the tnsB-dependent binding activity.

Original languageEnglish (US)
Pages (from-to)7807-7811
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number22
DOIs
StatePublished - Nov 1987
Externally publishedYes

ASJC Scopus subject areas

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