Identification of a Torpedo homolog of Sam68 that interacts with the synapse organizing protein rapsyn

Eric T. Fung, Anthony Lanahan, Paul Worley, Richard L. Huganir

Research output: Contribution to journalArticle

Abstract

Nicotinic acetylcholine receptors (nAChRs) are initially expressed diffusely on the surface of myotubes and, in response to neuronally derived factors, cluster at the endplate to a final concentration of approximately 10 000/μm2. The synaptic peripheral membrane protein rapsyn has been shown to mediate clustering of nAChRs in several systems. Here we describe the use of the yeast two-hybrid system to identify proteins that can interact with rapsyn. One of the clones we have identified is a Torpedo californica homolog of the Src-associated in mitosis protein (Sam68). We further show that Sam68, like rapsyn, is localized at the neuromuscular junction. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)29-33
Number of pages5
JournalFEBS Letters
Volume437
Issue number1-2
DOIs
StatePublished - Oct 16 1998
Externally publishedYes

Keywords

  • Neuromuscular junction
  • Sam68

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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