Abstract
Nicotinic acetylcholine receptors (nAChRs) are initially expressed diffusely on the surface of myotubes and, in response to neuronally derived factors, cluster at the endplate to a final concentration of approximately 10 000/μm2. The synaptic peripheral membrane protein rapsyn has been shown to mediate clustering of nAChRs in several systems. Here we describe the use of the yeast two-hybrid system to identify proteins that can interact with rapsyn. One of the clones we have identified is a Torpedo californica homolog of the Src-associated in mitosis protein (Sam68). We further show that Sam68, like rapsyn, is localized at the neuromuscular junction. Copyright (C) 1998 Federation of European Biochemical Societies.
Original language | English (US) |
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Pages (from-to) | 29-33 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 437 |
Issue number | 1-2 |
DOIs | |
State | Published - Oct 16 1998 |
Externally published | Yes |
Keywords
- Neuromuscular junction
- Sam68
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology