Identification of a subgroup of glycosylphosphatidylinositol-anchored tryptases

G. William Wong, Richard L. Stevens

Research output: Contribution to journalArticle

Abstract

The tryptase locus on mouse chromosome 17A3.3 contains 13 genes that encode enzymatically active serine proteases with different tissue expression profiles and substrate specificities. Mouse mast cell protease (mMCP) 6, mMCP-7, mMCP-11/protease serine member S (Prss) 34, tryptase 6/Prss33, tryptase ε/Prss22, implantation serine protease (Isp) 1/Prss28, and Isp-2 are constitutively exocytosed enzymes. We now demonstrate that tryptase 5/Prss32, pancreasin/Prss27, and testis serine protease-1 are inserted into plasma membranes via glycosylphosphatidylinositol (GPI) anchors analogous to Prss21, and that these serine proteases can be released from the cell's surface by a phosphatidylinositol-specific phospholipase C. These data suggest that the C-terminal residues play key roles in determining where tryptases compartmentalize in cells. GPI-anchored proteins are targeted to lipid rafts. Thus, our identification of a number of GPI-anchored tryptases whose genes reside at mouse chromosome 17A3.3 also implicates important biological functions for this new family of serine proteases on the surfaces of cells.

Original languageEnglish (US)
Pages (from-to)579-584
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume336
Issue number2
DOIs
StatePublished - Oct 21 2005
Externally publishedYes

Keywords

  • GPI
  • Membrane protease
  • Mouse chromosome 17
  • Phospholipase C
  • Prss
  • Serine protease
  • Tryptase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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