Identification of a redox-sensitive cysteine in GCP60 that regulates its interaction with Golgin-160

Juan Sbodio, Carolyn E Machamer

Research output: Contribution to journalArticle

Abstract

Golgin-160 is ubiquitously expressed in vertebrates. It localizes to the cytoplasmic side of the Golgi and has a large C-terminal coiled-coil domain. The noncoiled-coil N-terminal head domain contains Golgi targeting information, a cryptic nuclear localization signal, and three caspase cleavage sites. Caspase cleavage of the golgin-160 head domain generates different fragments that can translocate to the nucleus by exposing the nuclear localization signal. We have previously shown that GCP60, a Golgi resident protein, interacts weakly with the golgin-160 head domain but has a strong interaction with one of the caspase-generated golgin-160 fragments (residues 140-311). This preferential interaction increases the Golgi retention of the golgin-160 fragment in cells overexpressing GCP60. Here we studied the interaction of golgin-160-(140-311) with GCP60 and identified a single cysteine residue in GCP60 (Cys-463) that is critical for the interaction of the two proteins. Mutation of the cysteine blocked the interaction in vitro and disrupted the ability to retain the golgin-160 fragment at the Golgi in cells. We also found that Cys-463 is redox-sensitive; in its reduced form, interaction with golgin-160 was diminished or abolished, whereas oxidation of the Cys-463 by hydrogen peroxide restored the interaction. In addition, incubation with a nitric oxide donor promoted this interaction in vitro. These findings suggest that nuclear translocation of golgin-160-(140-311) is a highly coordinated event regulated not only by cleavage of the golgin-160 head but also by the oxidation state of GCP60.

Original languageEnglish (US)
Pages (from-to)29874-29881
Number of pages8
JournalJournal of Biological Chemistry
Volume282
Issue number41
DOIs
StatePublished - Oct 12 2007

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Caspases
Oxidation-Reduction
Cysteine
Head
Nuclear Localization Signals
Oxidation
Nitric Oxide Donors
Hydrogen Peroxide
Proteins
Vertebrates
Mutation
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification of a redox-sensitive cysteine in GCP60 that regulates its interaction with Golgin-160. / Sbodio, Juan; Machamer, Carolyn E.

In: Journal of Biological Chemistry, Vol. 282, No. 41, 12.10.2007, p. 29874-29881.

Research output: Contribution to journalArticle

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