TY - JOUR
T1 - Identification of a recurrent pattern of false-positivity by Luminex HLA MHC class I single antigen bead testing
AU - Dean, Christina L.
AU - Krummey, Scott M.
AU - Gebel, Howard M.
AU - Bray, Robert A.
AU - Sullivan, Harold C.
N1 - Publisher Copyright:
© 2020 American Society for Histocompatibility and Immunogenetics
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Previously, a distinct MHC class II Luminex-single antigen bead (SAB) pattern was described and attributed to antibodies targeting denatured antigens. In this study, we describe a distinct MHC class I reactivity pattern observed in 1.8% (105/5992) of samples resulted in 2017. The pattern displays reactivity to the following Luminex-SABs: HLA-A*33:03, A*36:01, A*80:01, B*54:01, B*53:01, C*06:02, C*07:02, C*18:02, C*14:02, C*03:03, C*03:04, and C*15:02. This pattern was identified in patients with no sensitization history, negative FlowPRA results, and antibody to self-antigen(s). Epitope analysis failed to reveal a common determinant(s) to explain this pattern of reactivity. Additionally, we found this pattern to be prevalent in female patients (62%) and also those with systemic lupus erythematosus (62%). Given these findings, we speculate this pattern likely represents false-positive reactivity, possibly due to antibody targeting denatured antigens or a specific peptide, molecular mimicry, autoimmunity, or a combination thereof.
AB - Previously, a distinct MHC class II Luminex-single antigen bead (SAB) pattern was described and attributed to antibodies targeting denatured antigens. In this study, we describe a distinct MHC class I reactivity pattern observed in 1.8% (105/5992) of samples resulted in 2017. The pattern displays reactivity to the following Luminex-SABs: HLA-A*33:03, A*36:01, A*80:01, B*54:01, B*53:01, C*06:02, C*07:02, C*18:02, C*14:02, C*03:03, C*03:04, and C*15:02. This pattern was identified in patients with no sensitization history, negative FlowPRA results, and antibody to self-antigen(s). Epitope analysis failed to reveal a common determinant(s) to explain this pattern of reactivity. Additionally, we found this pattern to be prevalent in female patients (62%) and also those with systemic lupus erythematosus (62%). Given these findings, we speculate this pattern likely represents false-positive reactivity, possibly due to antibody targeting denatured antigens or a specific peptide, molecular mimicry, autoimmunity, or a combination thereof.
KW - HLA antibody
KW - Luminex assay
KW - MHC class I
KW - Single antigen bead
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U2 - 10.1016/j.humimm.2019.12.006
DO - 10.1016/j.humimm.2019.12.006
M3 - Article
C2 - 31917024
AN - SCOPUS:85077630785
VL - 81
SP - 73
EP - 78
JO - Human Immunology
JF - Human Immunology
SN - 0198-8859
IS - 2-3
ER -