Identification of a recurrent pattern of false-positivity by Luminex HLA MHC class I single antigen bead testing

Christina L. Dean, Scott M. Krummey, Howard M. Gebel, Robert A. Bray, Harold C. Sullivan

Research output: Contribution to journalArticlepeer-review

Abstract

Previously, a distinct MHC class II Luminex-single antigen bead (SAB) pattern was described and attributed to antibodies targeting denatured antigens. In this study, we describe a distinct MHC class I reactivity pattern observed in 1.8% (105/5992) of samples resulted in 2017. The pattern displays reactivity to the following Luminex-SABs: HLA-A*33:03, A*36:01, A*80:01, B*54:01, B*53:01, C*06:02, C*07:02, C*18:02, C*14:02, C*03:03, C*03:04, and C*15:02. This pattern was identified in patients with no sensitization history, negative FlowPRA results, and antibody to self-antigen(s). Epitope analysis failed to reveal a common determinant(s) to explain this pattern of reactivity. Additionally, we found this pattern to be prevalent in female patients (62%) and also those with systemic lupus erythematosus (62%). Given these findings, we speculate this pattern likely represents false-positive reactivity, possibly due to antibody targeting denatured antigens or a specific peptide, molecular mimicry, autoimmunity, or a combination thereof.

Original languageEnglish (US)
Pages (from-to)73-78
Number of pages6
JournalHuman Immunology
Volume81
Issue number2-3
DOIs
StatePublished - Feb 1 2020
Externally publishedYes

Keywords

  • HLA antibody
  • Luminex assay
  • MHC class I
  • Single antigen bead

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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