TY - JOUR
T1 - Identification of a primate cytomegalovirus group-common protein antigen
AU - Weiner, Diana
AU - Gibson, Wade
N1 - Funding Information:
These studies were aided by Grants AI 13718 and AI 15959 from the National Institute for Allergy and Infectious Diseases, and Grant 1-613 from the National Foundation for Birth Defects. Diana Weiner is a predoctoral fellow in the Biochemistry, Cellular and Molecular Biology Training Program and was supported by National Science Foundation Fellowship SPl-7922316.
PY - 1981/11
Y1 - 1981/11
N2 - A monospecific rabbit antiserum has been prepared to the denatured 145K major capsid protein of strain Colburn cytomegalovirus (CMV). Using this antiserum to analyze proteins electrophoretically transferred to nitrocellulose, it was found that infected cell preparations from all primate CMVs examined contain a single cross-reactive protein band that ranged in molecular weight from 144K to 153K, depending upon the isolate. No reactivity was observed when similar preparations of herpes simplex virus types 1 (HSV-1) and 2 (HSV-2), and herpes zoster virus (HZV) were tested, suggesting that this antiserum may be CMV group-specific. A second antiserum prepared to the denatured Colburn 66K matrix protein was found to be more selective in its reactivity toward other simian CMVs, and without reactivity to human CMV preparations.
AB - A monospecific rabbit antiserum has been prepared to the denatured 145K major capsid protein of strain Colburn cytomegalovirus (CMV). Using this antiserum to analyze proteins electrophoretically transferred to nitrocellulose, it was found that infected cell preparations from all primate CMVs examined contain a single cross-reactive protein band that ranged in molecular weight from 144K to 153K, depending upon the isolate. No reactivity was observed when similar preparations of herpes simplex virus types 1 (HSV-1) and 2 (HSV-2), and herpes zoster virus (HZV) were tested, suggesting that this antiserum may be CMV group-specific. A second antiserum prepared to the denatured Colburn 66K matrix protein was found to be more selective in its reactivity toward other simian CMVs, and without reactivity to human CMV preparations.
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U2 - 10.1016/0042-6822(81)90100-8
DO - 10.1016/0042-6822(81)90100-8
M3 - Article
C2 - 6270897
AN - SCOPUS:0019637544
SN - 0042-6822
VL - 115
SP - 182
EP - 191
JO - Virology
JF - Virology
IS - 1
ER -