Identification of a peroxisome proliferator responsive element (PPRE)-like cis-element in mouse plasminogen activator inhibitor-1 gene promoter

Jie Gen Chen, Xi Li, Hai Yan Huang, Hong Lei Liu, De Guo Liu, Tan Jing Song, Chun Gu Ma, Duan Ma, Hou Yan Song, Qi Qun Tang

Research output: Contribution to journalArticle

Abstract

PAI-1 is expressed and secreted by adipose tissue which may mediate the pathogenesis of obesity-associated cardiovascular complications. Evidence is presented in this report that PAI-1 is not expressed by preadipocyte, but significantly induced during 3T3-L1 adipocyte differentiation and the PAI-1 expression correlates with the induction of peroxisome proliferator-activated receptor γ (PPARγ). A peroxisome proliferator responsive element (PPRE)-like cis-element (-206TCCCCCATGCCCT-194) is identified in the mouse PAI-1 gene promoter by electrophoretic mobility shift assay (EMSA) combined with transient transfection experiments; the PPRE-like cis-element forms a specific DNA-protein complex only with adipocyte nuclear extracts, not with preadipocyte nuclear extracts; the DNA-protein complex can be totally competed away by non-labeled consensus PPRE, and can be supershifted with PPARγ antibody. Mutation of this PPRE-like cis-element can abolish the transactivation of mouse PAI-1 promoter mediated by PPARγ. Specific PPARγ ligand Pioglitazone can significantly induce the PAI-1 expression, and stimulate the secretion of PAI-1 into medium.

Original languageEnglish (US)
Pages (from-to)821-826
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume347
Issue number3
DOIs
StatePublished - Sep 1 2006
Externally publishedYes

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Keywords

  • 3T3-L1
  • Peroxisome proliferator responsive element
  • Peroxisome proliferator-activated receptor γ
  • Pioglitazone
  • Plasminogen activator inhibitor-1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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