Abstract
PAI-1 is expressed and secreted by adipose tissue which may mediate the pathogenesis of obesity-associated cardiovascular complications. Evidence is presented in this report that PAI-1 is not expressed by preadipocyte, but significantly induced during 3T3-L1 adipocyte differentiation and the PAI-1 expression correlates with the induction of peroxisome proliferator-activated receptor γ (PPARγ). A peroxisome proliferator responsive element (PPRE)-like cis-element (-206TCCCCCATGCCCT-194) is identified in the mouse PAI-1 gene promoter by electrophoretic mobility shift assay (EMSA) combined with transient transfection experiments; the PPRE-like cis-element forms a specific DNA-protein complex only with adipocyte nuclear extracts, not with preadipocyte nuclear extracts; the DNA-protein complex can be totally competed away by non-labeled consensus PPRE, and can be supershifted with PPARγ antibody. Mutation of this PPRE-like cis-element can abolish the transactivation of mouse PAI-1 promoter mediated by PPARγ. Specific PPARγ ligand Pioglitazone can significantly induce the PAI-1 expression, and stimulate the secretion of PAI-1 into medium.
Original language | English (US) |
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Pages (from-to) | 821-826 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 347 |
Issue number | 3 |
DOIs | |
State | Published - Sep 1 2006 |
Externally published | Yes |
Keywords
- 3T3-L1
- Peroxisome proliferator responsive element
- Peroxisome proliferator-activated receptor γ
- Pioglitazone
- Plasminogen activator inhibitor-1
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology