Identification of a novel gene encoding a p53-associated protein

R. Zhou, H. Wen, S. Z. Ao

Research output: Contribution to journalArticlepeer-review

Abstract

p53 exerts important physiological functions in cell-cycle control, gene regulation, cell differentiation, apoptosis and tumor suppression by interacting with many cellular proteins. Using the yeast two-hybrid system, we screened a HeLa cDNA library and identified a novel gene encoding a p53-binding protein (p53BP3). The full-length cDNA of p53BP3 was isolated from a HeLaλgt10 cDNA library. This predicted protein was composed of 815 amino acids. Sequence analysis indicated that p53BP3 contained two bipartite nuclear localization signals and was confirmed to be a nuclear protein. FISH mapping results showed that this novel gene was located at human chromosome 12, region p11.2-p12.1. Northern blot analysis suggested that p53BP3 was broadly expressed in human tissues. A further study showed that p53BP3 had a homologue in mouse. (C) 1999 Elsevier Science B.V. All rights reserved.

Original languageEnglish (US)
Pages (from-to)93-101
Number of pages9
JournalGene
Volume235
Issue number1-2
DOIs
StatePublished - Jul 22 1999
Externally publishedYes

Keywords

  • Chromosome 12p11.2-p12.1
  • FISH
  • Leucine zipper protein
  • Yeast two-hybrid system
  • p53
  • p53-binding-protein

ASJC Scopus subject areas

  • Genetics

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