Identification of a novel gene encoding a p53-associated protein

Rui Zhou, H. Wen, S. Z. Ao

Research output: Contribution to journalArticle

Abstract

p53 exerts important physiological functions in cell-cycle control, gene regulation, cell differentiation, apoptosis and tumor suppression by interacting with many cellular proteins. Using the yeast two-hybrid system, we screened a HeLa cDNA library and identified a novel gene encoding a p53-binding protein (p53BP3). The full-length cDNA of p53BP3 was isolated from a HeLaλgt10 cDNA library. This predicted protein was composed of 815 amino acids. Sequence analysis indicated that p53BP3 contained two bipartite nuclear localization signals and was confirmed to be a nuclear protein. FISH mapping results showed that this novel gene was located at human chromosome 12, region p11.2-p12.1. Northern blot analysis suggested that p53BP3 was broadly expressed in human tissues. A further study showed that p53BP3 had a homologue in mouse. (C) 1999 Elsevier Science B.V. All rights reserved.

Original languageEnglish (US)
Pages (from-to)93-101
Number of pages9
JournalGene
Volume235
Issue number1-2
DOIs
StatePublished - Jul 22 1999
Externally publishedYes

Fingerprint

Gene Library
cdc Genes
Nuclear Localization Signals
Chromosomes, Human, Pair 12
Two-Hybrid System Techniques
Human Chromosomes
Nuclear Proteins
Cell Cycle Checkpoints
Northern Blotting
Genes
Sequence Analysis
Cell Differentiation
Carrier Proteins
Proteins
Complementary DNA
Apoptosis
Amino Acids
Neoplasms

Keywords

  • Chromosome 12p11.2-p12.1
  • FISH
  • Leucine zipper protein
  • p53
  • p53-binding-protein
  • Yeast two-hybrid system

ASJC Scopus subject areas

  • Genetics

Cite this

Identification of a novel gene encoding a p53-associated protein. / Zhou, Rui; Wen, H.; Ao, S. Z.

In: Gene, Vol. 235, No. 1-2, 22.07.1999, p. 93-101.

Research output: Contribution to journalArticle

Zhou, Rui ; Wen, H. ; Ao, S. Z. / Identification of a novel gene encoding a p53-associated protein. In: Gene. 1999 ; Vol. 235, No. 1-2. pp. 93-101.
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