Identification of a nonprocessive telomerase activity from mouse cells

Karen R. Prowse, Ariel A. Avilion, Carol W Greider

Research output: Contribution to journalArticle

Abstract

Telomerase activity was identified in extracts from several different mouse cell lines. Addition of telomeric TTAGGG repeats was specific to telomeric oligonucleotide primers and sensitive to pretreatment with RNase A. In contrast to the hundreds of repeats synthesized by the human and Tetrahymena telomerase enzymes in vitro, mouse telomerase synthesized only one or two TTAGGG repeats onto telomeric primers. The products observed after elongation of primers with circularly permuted (TTAGGG)3 sequences and after chain termination with ddATP or ddTTP indicated that mouse telomerase pauses after the addition of the first dG residue in the sequence TTAGGG. The short length of the products synthesized by mouse telomerase was not due to a diffusible inhibitor in the mouse extract, because the human telomerase continued to synthesize long products when mixed with mouse fractions. Primer challenge experiments showed that the human enzyme synthesized long TTAGGG repeats processively in vitro, whereas the mouse telomerase appeared to be much less processive. The identification of short telomerase reaction products in mouse extracts suggests that extracts from other organisms may also generate only short products. This knowledge may aid in the identification of telomerase activity in organisms where activity has not yet been detected.

Original languageEnglish (US)
Pages (from-to)1493-1497
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number4
StatePublished - Feb 15 1993
Externally publishedYes

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Telomerase
Tetrahymena
Pancreatic Ribonuclease
DNA Primers
Enzymes
Cell Line

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Identification of a nonprocessive telomerase activity from mouse cells. / Prowse, Karen R.; Avilion, Ariel A.; Greider, Carol W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 4, 15.02.1993, p. 1493-1497.

Research output: Contribution to journalArticle

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