Identification of a human Ia antigen that is different from HLA-DR and DC antigens

M. W. Makgobat, J. E K Hildreth, A. J. McMichael

Research output: Contribution to journalArticle

Abstract

A monoclonal antibody, MHM4, identified a cell surface antigen present on B cells and not resting T cells. It precipitated two polypeptide chains of 34 000 and 28 000 daltons from B lymphoblastoid cells. This antibody bound to all B-cell lines tested, except those homozygous for HLA-DR7. Saturation binding assays and Scatchard plots of MHM4 binding to cells that did not carry HLA-DR7 indicated that this antibody bound less than the total surface Ia antigen. When the antibody was competed with eight other HLA-D-specific antibodies, the epitope recognized was shown to be distinct. Two-dimensional gel analysis revealed that a simple pattern of spots was precipitated, unlike the complex patterns obtained with other HLA-D-specific antibodies. The α and β spots were different from those precipitated by HLA-DR- or DC-specific antibodies. It is argued that the MHM4 antigen is the product of an HLA locus that is distinct from HLA-DR and DC. Its relationship with HLA-SI3 is discussed.

Original languageEnglish (US)
Pages (from-to)623-635
Number of pages13
JournalImmunogenetics
Volume17
Issue number6
DOIs
StatePublished - 1983
Externally publishedYes

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HLA-DQ Antigens
Forensic Anthropology
Histocompatibility Antigens Class II
HLA-DR Antigens
Antibodies
HLA-DR7 Antigen
HLA-D Antigens
Surface Antigens
B-Lymphocytes
Epitopes
Gels
Monoclonal Antibodies
T-Lymphocytes
Antigens
Cell Line
Peptides

ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

Identification of a human Ia antigen that is different from HLA-DR and DC antigens. / Makgobat, M. W.; Hildreth, J. E K; McMichael, A. J.

In: Immunogenetics, Vol. 17, No. 6, 1983, p. 623-635.

Research output: Contribution to journalArticle

Makgobat, M. W. ; Hildreth, J. E K ; McMichael, A. J. / Identification of a human Ia antigen that is different from HLA-DR and DC antigens. In: Immunogenetics. 1983 ; Vol. 17, No. 6. pp. 623-635.
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