We have identified a sequence in the TCR β2 locus that is homologous to the κB site in the Ig κ light chain enhancer. This element, TCRβ-B, is located in the vicinity of previously identified T cell-specific DNase1 hypersensitive sites. Transfection analysis shows that a 60-bp fragment encompassing this site is preferentially active in T cells stimulated with phorbol esters or the HTLV-1 tax gene product compared with a B cell line that constitutively expresses NF-κB. Our results provide the first evidence for transcriptional regulatory sequences residing within the Jβ2-Cβ2 intron and suggest the possible involvement of these sequences in modulation of TCRβ gene expression upon cellular activation.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of Experimental Medicine|
|State||Published - 1989|
ASJC Scopus subject areas