Identification of a functional homolog of the yeast copper homeostasis gene ATX1 from arabidopsis

Edward Himelblau, Helena Mira, Su Ju Lin, Valeria L Culotta, Lola Peñarrubia, Richard M. Amasino

Research output: Contribution to journalArticle

Abstract

A cDNA clone encoding a homolog of the yeast (Saccharomyces cerevisiae) gene Anti-oxidant 1 (ATX1) has been identified from Arabidopsis. This gene, referred to as Copper CHaperone (CCH), encodes a protein that is 36% identical to the amino acid sequence of ATX1 and has a 48-amino acid extension at the C-terminal end, which is absent from ATX1 homologs identified in animals. ATX1-deficient yeast (atxl) displayed a loss of high-affinity iron uptake. Expression of CCH in the atxl strain restored high-affinity iron uptake, demonstrating that CCH is a functional homolog of ATX1. When overexpressed in yeast lacking the Superoxide dismutase gene SOD1, both ATX1 and CCH protected the cell from the reactive oxygen toxicity that results from Superoxide dismutase deficiency. CCH was unable to rescue the sod1 phenotype in the absence of copper, indicating that CCH function is copper dependent. In Arabidopsis CCH mRNA is present in the root, leaf, and inflorescence and is up-regulated 7-fold in leaves undergoing senescence. In plants treated with 800 nL/L ozone for 30 min, CCH mRNA levels increased by 30%. In excised leaves and whole plants treated with high levels of exogenous CuSO4, CCH mRNA levels decreased, indicating that CCH is regulated differently than characterized metallothionein proteins in Arabidopsis.

Original languageEnglish (US)
Pages (from-to)1227-1234
Number of pages8
JournalPlant Physiology
Volume117
Issue number4
StatePublished - 1998

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Arabidopsis
Oxidants
oxidants
Copper
homeostasis
Homeostasis
Yeasts
copper
yeasts
Genes
genes
Messenger RNA
Superoxide Dismutase
superoxide dismutase
Iron
Arabidopsis Proteins
iron
uptake mechanisms
leaves
Inflorescence

ASJC Scopus subject areas

  • Plant Science

Cite this

Himelblau, E., Mira, H., Lin, S. J., Culotta, V. L., Peñarrubia, L., & Amasino, R. M. (1998). Identification of a functional homolog of the yeast copper homeostasis gene ATX1 from arabidopsis. Plant Physiology, 117(4), 1227-1234.

Identification of a functional homolog of the yeast copper homeostasis gene ATX1 from arabidopsis. / Himelblau, Edward; Mira, Helena; Lin, Su Ju; Culotta, Valeria L; Peñarrubia, Lola; Amasino, Richard M.

In: Plant Physiology, Vol. 117, No. 4, 1998, p. 1227-1234.

Research output: Contribution to journalArticle

Himelblau, E, Mira, H, Lin, SJ, Culotta, VL, Peñarrubia, L & Amasino, RM 1998, 'Identification of a functional homolog of the yeast copper homeostasis gene ATX1 from arabidopsis', Plant Physiology, vol. 117, no. 4, pp. 1227-1234.
Himelblau, Edward ; Mira, Helena ; Lin, Su Ju ; Culotta, Valeria L ; Peñarrubia, Lola ; Amasino, Richard M. / Identification of a functional homolog of the yeast copper homeostasis gene ATX1 from arabidopsis. In: Plant Physiology. 1998 ; Vol. 117, No. 4. pp. 1227-1234.
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