Identification of 7-Carboxymethylguanine in DNA from Pancreatic Acinar Cells Exposed to Azaserine

Joanne Zurlo, Thomas J. Curnhav, Raymond Hiley, Daniel S. Longnecker, Joanne Zurlo

Research output: Contribution to journalArticle

Abstract

Studies were undertaken to determine the identity of an azaserine:DNA adduct. The most probable adduct, 7-carbox-ymethylguanine, was synthesized. DNA isolated from pancreatic acinar cells treated in culture with [14C]azaserine was hydrolyzed under neutral conditions to liberate N-alkylated purines. The neutral hydrolysate was subjected to high-per-formance liquid chromatography along with the synthetic standard. One of the radioactive peaks from the treated DNA was found to cochromatograph with 7-carboxymethylguanine in three systems: reverse phase; anion exchange; and ion pair reverse phase. These results suggest that azaserine metabolism in acinar cells results in carboxymethylation of DNA, supporting previously proposed models of azaserine degradation.

Original languageEnglish (US)
Pages (from-to)1286-1288
Number of pages3
JournalCancer Research
Volume42
Issue number4
StatePublished - Apr 1 1982

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Zurlo, J., Curnhav, T. J., Hiley, R., Longnecker, D. S., & Zurlo, J. (1982). Identification of 7-Carboxymethylguanine in DNA from Pancreatic Acinar Cells Exposed to Azaserine. Cancer Research, 42(4), 1286-1288.