TY - JOUR
T1 - Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru
AU - Xiao, Lihua
AU - Bern, Caryn
AU - Limor, Josef
AU - Sulaiman, Irshad
AU - Roberts, Jacquelin
AU - Checkley, William
AU - Cabrera, Lilia
AU - Gilman, Robert H.
AU - Lal, Altaf A.
N1 - Funding Information:
Financial support: National Institutes of Health (1-UO1-AI-35894-01);molecular work supported in part by funding from the Opportunistic Infectious Diseases program of the Centers for Disease Control and Prevention.
PY - 2001/2/1
Y1 - 2001/2/1
N2 - Cryptosporidium parvum is usually considered to be the pathogen responsible for human cryptosporidiosis. We genotyped Cryptosporidium in 132 stool specimens from 80 Peruvian children, representing 85 infection episodes, using techniques that differentiate Cryptosporidium species and C. parvum genotypes. Five types of Cryptosporidium were identified: C. parvum human (67), bovine (8), and dog (2) genotypes, C. meleagridis (7), and C. fells (1). Twenty-five (29%) of the 85 infection episodes were associated with diarrhea. There was no significant difference in age, antecedent stunting, percentage with diarrhea, or duration of diarrhea for episodes with human genotype, compared with those of zoonotic Cryptosporidium. Duration of oocyst shedding was longer for human genotype than for zoonotic Cryptosporidium (mean, 13.9 days and 6.4 days, respectively; P = .004). Serum samples from 8 children with C. meleagridis, C. felis, or C. parvum dog genotype were tested for anti-human immunodeficiency virus (HIV) type 1 antibodies; all were found to be negative. Contrary to common belief, novel Cryptosporidium species and C. parvum genotypes can infect HIV-negative children.
AB - Cryptosporidium parvum is usually considered to be the pathogen responsible for human cryptosporidiosis. We genotyped Cryptosporidium in 132 stool specimens from 80 Peruvian children, representing 85 infection episodes, using techniques that differentiate Cryptosporidium species and C. parvum genotypes. Five types of Cryptosporidium were identified: C. parvum human (67), bovine (8), and dog (2) genotypes, C. meleagridis (7), and C. fells (1). Twenty-five (29%) of the 85 infection episodes were associated with diarrhea. There was no significant difference in age, antecedent stunting, percentage with diarrhea, or duration of diarrhea for episodes with human genotype, compared with those of zoonotic Cryptosporidium. Duration of oocyst shedding was longer for human genotype than for zoonotic Cryptosporidium (mean, 13.9 days and 6.4 days, respectively; P = .004). Serum samples from 8 children with C. meleagridis, C. felis, or C. parvum dog genotype were tested for anti-human immunodeficiency virus (HIV) type 1 antibodies; all were found to be negative. Contrary to common belief, novel Cryptosporidium species and C. parvum genotypes can infect HIV-negative children.
UR - http://www.scopus.com/inward/record.url?scp=0035253664&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035253664&partnerID=8YFLogxK
U2 - 10.1086/318090
DO - 10.1086/318090
M3 - Article
C2 - 11133382
AN - SCOPUS:0035253664
SN - 0022-1899
VL - 183
SP - 492
EP - 497
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 3
ER -