Identification of α-fodrin as an autoantigen in experimental coronavirus retinopathy (ECOR)

Marian S. Chin, Laura C. Hooper, John J. Hooks, Barbara Detrick

Research output: Contribution to journalArticle

Abstract

The coronavirus, mouse hepatitis virus (MHV), JHM strain induces a biphasic disease in BALB/c mice that consists of an acute retinitis followed by progression to a chronic retinal degeneration with autoimmune reactivity. Retinal degeneration resistant CD-1 mice do not develop either the late phase or autoimmune reactivity. A mouse RPE/choroid DNA expression library was screened using sera from virus infected BALB/c mice. Two clones were identified, villin-2 protein and α-fodrin protein. α-Fodrin protein was used for further analysis and western blot reactivity was seen only in sera from virus infected BALB/c mice. CD4 T cells were shown to specifically react with MHV antigens and with α-fodrin protein. These studies clearly identified both antibody and CD4 T cell reactivities to α-fodrin in sera from virus infected, retinal degenerative susceptible BALB/c mice.

Original languageEnglish (US)
Pages (from-to)42-50
Number of pages9
JournalJournal of Neuroimmunology
Volume272
Issue number1-2
DOIs
StatePublished - Jan 1 2014

Keywords

  • Autoantibodies
  • Autoimmunity
  • Coronavirus
  • Retinal degeneration
  • α-Fodrin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Fingerprint Dive into the research topics of 'Identification of α-fodrin as an autoantigen in experimental coronavirus retinopathy (ECOR)'. Together they form a unique fingerprint.

  • Cite this