TY - JOUR
T1 - Identification, expression and characterization of rat isoforms of the serum response factor (SRF) coactivator MKL1
AU - Ishikawa, Mitsuru
AU - Shiota, Jun
AU - Ishibashi, Yuta
AU - Hakamata, Tomoyuki
AU - Shoji, Shizuku
AU - Fukuchi, Mamoru
AU - Tsuda, Masaaki
AU - Shirao, Tomoaki
AU - Sekino, Yuko
AU - Ohtsuka, Toshihisa
AU - Baraban, Jay M.
AU - Tabuchi, Akiko
N1 - Funding Information:
This study was supported by a grant-in aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (Project No. 17790055, 19790052 and 22590080, A.T.; 20390023, M.T.; 23890065, M.I. ) and by research grants from the Hayashi Memorial Foundation for Female Natural Scientists (A.T.), Foundation of the first Bank of Toyama (A.T.), Takeda Science Foundation (A.T.), Narishige Neuroscience Research Foundation (A.T.) and the Research Foundation for Pharmaceutical Sciences (A.T.).
PY - 2013
Y1 - 2013
N2 - Megakaryoblastic leukemia 1 (MKL1) is a member of the MKL family of serum response factor (SRF) coactivators. Here we have identified three rat MKL1 transcripts: two are homologues of mouse MKL1 transcripts, full-length MKL1 (FLMKL1) and basic, SAP, and coiled-coil domains (BSAC), the third is a novel transcript, MKL1. elongated derivative of yield (MELODY). These rat MKL1 transcripts are differentially expressed in a wide variety of tissues with highest levels in testis and brain. During brain development, these transcripts display differential patterns of expression. The FLMKL1 transcript encodes two isoforms that utilize distinct translation start sites. The longer form possesses three actin-binding RPXXXEL (RPEL) motifs and the shorter form, MKL1met only has two RPEL motifs. All four rat MKL1 isoforms, FLMKL1, BSAC, MKL1met and MELODY increased SRF-mediated transcription, but not CREB-mediated transcription. Accordingly, the differential expression of MKL1 isoforms may help fine-tune gene expression during brain development.
AB - Megakaryoblastic leukemia 1 (MKL1) is a member of the MKL family of serum response factor (SRF) coactivators. Here we have identified three rat MKL1 transcripts: two are homologues of mouse MKL1 transcripts, full-length MKL1 (FLMKL1) and basic, SAP, and coiled-coil domains (BSAC), the third is a novel transcript, MKL1. elongated derivative of yield (MELODY). These rat MKL1 transcripts are differentially expressed in a wide variety of tissues with highest levels in testis and brain. During brain development, these transcripts display differential patterns of expression. The FLMKL1 transcript encodes two isoforms that utilize distinct translation start sites. The longer form possesses three actin-binding RPXXXEL (RPEL) motifs and the shorter form, MKL1met only has two RPEL motifs. All four rat MKL1 isoforms, FLMKL1, BSAC, MKL1met and MELODY increased SRF-mediated transcription, but not CREB-mediated transcription. Accordingly, the differential expression of MKL1 isoforms may help fine-tune gene expression during brain development.
KW - Alternative promoter
KW - Megakaryoblastic leukemia
KW - Serum response factor
KW - Transcript
KW - Transcriptional coactivator
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U2 - 10.1016/j.fob.2013.09.001
DO - 10.1016/j.fob.2013.09.001
M3 - Article
C2 - 24251100
AN - SCOPUS:84884323988
SN - 2211-5463
VL - 3
SP - 387
EP - 393
JO - FEBS Open Bio
JF - FEBS Open Bio
ER -