Identification, cloning, and developmental expression of hepatoma-derived growth factor in the developing rat heart

Research output: Contribution to journalArticle

Abstract

Hepatoma derived growth factor (HDGF) was identified as a developmentally regulated cardiac gene by mRNA differential display using 12-day rat fetal conotruncus vs. newborn aorta. The full-length rat HDGF cDNA was cloned from a rat fetal heart cDNA library and found to be 94 and 88% homologous to the mouse and human sequence, respectively. The rat sequence, like the human and mouse, contains a highly conserved amino portion and putative bi-partite nuclear localization sequence. By Northern analysis, HDGF is highly expressed in the fetal conotruncus, heart, kidney, brain, and gut. By immunocytochemistry, HDGF was first detected only in atrial myocytes, hind gut epithelia, and notochord of the E10 rat with a nuclear expression pattern. By E12, expression had broadened to include the ventricular myocytes, endocardial cells, and cells of the ventricular outflow tract. HDGF is unique in that it is the first described nuclear targeted growth factor in the developing heart. The early expression of HDGF in embryonic heart and fetal gut suggests that HDGF may play a role in cardiovascular growth and differentiation.

Original languageEnglish (US)
Pages (from-to)450-458
Number of pages9
JournalDevelopmental Dynamics
Volume222
Issue number3
DOIs
StatePublished - Nov 20 2001
Externally publishedYes

Keywords

  • Brain
  • Growth factors
  • Gut
  • Heart development
  • Kidney

ASJC Scopus subject areas

  • Developmental Biology

Fingerprint Dive into the research topics of 'Identification, cloning, and developmental expression of hepatoma-derived growth factor in the developing rat heart'. Together they form a unique fingerprint.

  • Cite this