TY - JOUR
T1 - Identification by dna hybridization of enterotoxigenic escherichia coli in a longitudinal study of villages in thailand
AU - Echeverria, Peter
AU - Seriwatana, Jitvimol
AU - Taylor, David N.
AU - Tirapat, Chalard
AU - Chaicumpa, Wanpen M.
AU - Rowe, Bernard
N1 - Funding Information:
Received for publication April 23, 1984, and in revised form July 23, 1984. This work was supported in part by contract C6-181-81 from the World Health Organization. Please address requests for reprints to Dr. Peter Echeverria, AFRIMS, APO San Francisco, California 96346.
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1985/1
Y1 - 1985/1
N2 - Radioactively labeled enterotoxin genes were used to study the epidemiology of enterotoxigenic Escherichia coli infections in two Thai villages. When E. coli that were isolated from 674 specimens were fixed on nitrocellulose paper and examined for hybridization with E. coli enterotoxin gene probes in Bangkok, the technique had a sensitivity of 94% (31 of 33) and a specificity of 100% (641 of 641), when compared with tests of E. coli for enterotoxin production in the Y-l-adrenal cell and suckling-mouse assays. However, when the same specimens were fixed directly onto nitrocellulose paper at a field laboratory and transported to the reference laboratory for assay with the gene probes, 27 specimens that contained enterotoxigenic E. coli did not hybridize with the E. coli gene probes. Enterotoxigenic E. coli that hybridized with the LT, ST-H, and ST-P probes were identified in 10% (17 of 177) of villagers with diarrhea, 7% (8 of 108) of contacts of individuals with diarrhea caused by enterotoxigenic E. coli, and 3% (32 of 1, 199) of persons not associated with cases of diarrhea caused by enterotoxigenic E. coli. Enterotoxigenic E. coli that hybridized with the ST-II probe was not a cause of diarrhea. Alternative methods of retaining DNA on filters under field conditions are needed before this technique can be used for direct examination of specimens with enterotoxin gene probes.
AB - Radioactively labeled enterotoxin genes were used to study the epidemiology of enterotoxigenic Escherichia coli infections in two Thai villages. When E. coli that were isolated from 674 specimens were fixed on nitrocellulose paper and examined for hybridization with E. coli enterotoxin gene probes in Bangkok, the technique had a sensitivity of 94% (31 of 33) and a specificity of 100% (641 of 641), when compared with tests of E. coli for enterotoxin production in the Y-l-adrenal cell and suckling-mouse assays. However, when the same specimens were fixed directly onto nitrocellulose paper at a field laboratory and transported to the reference laboratory for assay with the gene probes, 27 specimens that contained enterotoxigenic E. coli did not hybridize with the E. coli gene probes. Enterotoxigenic E. coli that hybridized with the LT, ST-H, and ST-P probes were identified in 10% (17 of 177) of villagers with diarrhea, 7% (8 of 108) of contacts of individuals with diarrhea caused by enterotoxigenic E. coli, and 3% (32 of 1, 199) of persons not associated with cases of diarrhea caused by enterotoxigenic E. coli. Enterotoxigenic E. coli that hybridized with the ST-II probe was not a cause of diarrhea. Alternative methods of retaining DNA on filters under field conditions are needed before this technique can be used for direct examination of specimens with enterotoxin gene probes.
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U2 - 10.1093/infdis/151.1.124
DO - 10.1093/infdis/151.1.124
M3 - Article
C2 - 3880798
AN - SCOPUS:0021968518
SN - 0022-1899
VL - 151
SP - 124
EP - 130
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 1
ER -