Identification and verification of redox-sensitive proteins in arabidopsis Thaliana

Hai Wang, Shengbing Wang, Yiji Xia

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Environmental stresses often trigger rapid generation of reactive oxygen species (ROS) in plants. Excessive amount of ROS can cause damage to plant cells and thus need to be counteracted by cellular antioxidant systems. On the other hand, ROS also serve as signaling molecules that modulate various physiological responses and developmental processes. Signaling function of ROS is largely achieved through oxidative modifications of redox-sensitive proteins. Therefore, development of methods for high-throughput identification of redox-sensitive proteins and for verifying and characterizing their in vivo redox states is essential for advancing our understanding of ROS-mediated signaling pathways.

Original languageEnglish (US)
Title of host publicationPlant Signalling Networks
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages83-94
Number of pages12
ISBN (Print)9781617798085
DOIs
StatePublished - Jan 1 2012

Publication series

NameMethods in Molecular Biology
Volume876
ISSN (Print)1064-3745

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Keywords

  • Arabidopsis thaliana
  • Oxidative stress
  • Proteomics
  • Redox-sensitive proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Wang, H., Wang, S., & Xia, Y. (2012). Identification and verification of redox-sensitive proteins in arabidopsis Thaliana. In Plant Signalling Networks: Methods and Protocols (pp. 83-94). (Methods in Molecular Biology; Vol. 876). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-809-2_6