Identification and upregulation of galactose/N-acetylgalactosamine macrophage lectin in rat cardiac allografts with arteriosclerosis

Mary E. Russell, Ulrike Utans, Africa F. Wallace, Peng Liang, Robert J. Arceci, Morris J. Karnovsky, Lauri R. Wyner, Yukari Yamashita, Chi Tarn

Research output: Contribution to journalArticle

Abstract

Using differential mRNA display to uncover potential mediators associated with chronic rejection, we identified a cDNA fragment induced in Lewis to F344 rat cardiac allografts with arteriosclerosis but not Lewis syngrafts. The full-length cDNA (1.4 kb) isolated from a rat cardiac allograft cDNA library was 99% identical to galactose/N-acetylgalactosamine (Gal/GalNAc) macrophage lectin, a cell-surface receptor. This cDNA hybridized in Northern analysis with total RNA from eight cardiac allografts but not with host hearts, syngrafts, or other organs. There was a significant allograft- specific increase in transcript levels measured by reverse transcriptase PCR at days 7, 14, 28, and 75 in comparison with paired F344 host hearts (subject to same circulation but histologically normal), day-0 hearts, and syngrafts (P <0.008, n = 4 at each time). Transcript levels in cardiac allografts were higher than those in paired host spleens (a major source of inflammatory cells) (P <0.0001), indicating the localized nature of Gal/GalNAc lectin induction. By in situ hybridization and immunostaining, Gal/GalNAc lectin expression localized to a subset of inflammatory cells in cardiac allografts. These findings link Gal/GalNAc macrophage lectin to the chronic rejection process, as a possible mediator of macrophage infiltration.

Original languageEnglish (US)
Pages (from-to)722-730
Number of pages9
JournalJournal of Clinical Investigation
Volume94
Issue number2
StatePublished - Aug 1994
Externally publishedYes

Fingerprint

Acetylgalactosamine
Arteriosclerosis
Galactose
Lectins
Allografts
Up-Regulation
Macrophages
Complementary DNA
Matched-Pair Analysis
Inbred F344 Rats
Cell Surface Receptors
Gene Expression Profiling
Reverse Transcriptase Polymerase Chain Reaction
Gene Library
In Situ Hybridization
Spleen
RNA
Thomsen-Friedenreich antigen

Keywords

  • arteriosclerosis
  • asialoglycoproteins
  • cardiac transplantation
  • gene expression
  • rejection

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Russell, M. E., Utans, U., Wallace, A. F., Liang, P., Arceci, R. J., Karnovsky, M. J., ... Tarn, C. (1994). Identification and upregulation of galactose/N-acetylgalactosamine macrophage lectin in rat cardiac allografts with arteriosclerosis. Journal of Clinical Investigation, 94(2), 722-730.

Identification and upregulation of galactose/N-acetylgalactosamine macrophage lectin in rat cardiac allografts with arteriosclerosis. / Russell, Mary E.; Utans, Ulrike; Wallace, Africa F.; Liang, Peng; Arceci, Robert J.; Karnovsky, Morris J.; Wyner, Lauri R.; Yamashita, Yukari; Tarn, Chi.

In: Journal of Clinical Investigation, Vol. 94, No. 2, 08.1994, p. 722-730.

Research output: Contribution to journalArticle

Russell, ME, Utans, U, Wallace, AF, Liang, P, Arceci, RJ, Karnovsky, MJ, Wyner, LR, Yamashita, Y & Tarn, C 1994, 'Identification and upregulation of galactose/N-acetylgalactosamine macrophage lectin in rat cardiac allografts with arteriosclerosis', Journal of Clinical Investigation, vol. 94, no. 2, pp. 722-730.
Russell, Mary E. ; Utans, Ulrike ; Wallace, Africa F. ; Liang, Peng ; Arceci, Robert J. ; Karnovsky, Morris J. ; Wyner, Lauri R. ; Yamashita, Yukari ; Tarn, Chi. / Identification and upregulation of galactose/N-acetylgalactosamine macrophage lectin in rat cardiac allografts with arteriosclerosis. In: Journal of Clinical Investigation. 1994 ; Vol. 94, No. 2. pp. 722-730.
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