Identification and purification of the human myeloid differentiation antigen recognized by monoclonal antibody AHN-7

K. M. Skubitz, D. J. Weisdorf, Thomas August

Research output: Contribution to journalArticle

Abstract

This report describes the characterization and expression of a human myeloid differentiation antigen defined by use of an IgG1 monoclonal antibody (AHN-7). This antibody binds to many granulocytic precursors in normal marrow, to most but not all granulocyte-macrophage progenitors (CFU-GM), and to approximately half of nonlymphoid leukemia specimens. The protein antigens recognized by AHN-7 were purified from 35S-labelled HL-60 cells by antibody affinity column chromatography. The molecule reacting with AHN-7 was markedly heterogeneous, appearing as several forms ranging in pI from 4.5 to 6.4 with apparent molecular weights from 43,000 to 68,000. The molecules were not disulfide-linked. Proteins bearing the antigen were minor components of the plasma membrane. The antigen was expressed by normal human peripheral blood neutrophils, monocytes, eosinophils, and basophils, and weakly on a small percentage of lymphocytes; it was not detected in red blood cells, platelets, or the majority of lymphocytes. The antibody also bound to a variety of human myeloid leukemia cell lines but not to any lymphoid leukemia cell line tested. AHN-7 had no effect on several in vitro neutrophil functions tested.

Original languageEnglish (US)
Pages (from-to)104-110
Number of pages7
JournalJournal of Leukocyte Biology
Volume41
Issue number2
StatePublished - 1987
Externally publishedYes

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Forensic Anthropology
Differentiation Antigens
Granulocyte-Macrophage Progenitor Cells
Monoclonal Antibodies
Lymphocytes
Antigens
Neutrophils
Lymphoid Leukemia
Cell Line
Myeloid Leukemia
Antibody Affinity
Basophils
Antibodies
HL-60 Cells
Myeloid Cells
Affinity Chromatography
Eosinophils
Disulfides
Monocytes
Leukemia

ASJC Scopus subject areas

  • Cell Biology

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Identification and purification of the human myeloid differentiation antigen recognized by monoclonal antibody AHN-7. / Skubitz, K. M.; Weisdorf, D. J.; August, Thomas.

In: Journal of Leukocyte Biology, Vol. 41, No. 2, 1987, p. 104-110.

Research output: Contribution to journalArticle

Skubitz, K. M. ; Weisdorf, D. J. ; August, Thomas. / Identification and purification of the human myeloid differentiation antigen recognized by monoclonal antibody AHN-7. In: Journal of Leukocyte Biology. 1987 ; Vol. 41, No. 2. pp. 104-110.
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