Identification and partial purification of phosphatidylcholine phospholipase C from the cryptobiotic cysts of the fairy shrimp, Streptocephalus dichotomus

Research output: Contribution to journalArticle

Abstract

The occurrence of phosphatidyl choline specific phospholipase C (PC-PLC)(EC 3.1.4.3) which liberates diacylglycerol (DAG), an important second messenger molecule, has been reported for the first time from the cryptobiotic cysts of the fairy shrimp, Streptocephalus dichotomus. Since the cysts undergo a revival of metabolism and resumption of development upon hydration, the possible role of the enzyme, in these processes was investigated. The results revealed enzyme's presence and an increase in its activity with the advancement of cyst development. Further partial purification of the enzyme using a lipoprotein linked-Sepharose CL-4B column (an affinity chromatographic principle) was attempted. The purification of 19.3 fold with a yield of 80% was attained.

Original languageEnglish (US)
Pages (from-to)203-207
Number of pages5
JournalBiochemistry and Molecular Biology International
Volume36
Issue number1
StatePublished - 1995
Externally publishedYes

Fingerprint

Anostraca
Type C Phospholipases
Phosphatidylcholines
Purification
Cysts
Enzymes
Diglycerides
Second Messenger Systems
Metabolism
Hydration
Lipoproteins
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

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abstract = "The occurrence of phosphatidyl choline specific phospholipase C (PC-PLC)(EC 3.1.4.3) which liberates diacylglycerol (DAG), an important second messenger molecule, has been reported for the first time from the cryptobiotic cysts of the fairy shrimp, Streptocephalus dichotomus. Since the cysts undergo a revival of metabolism and resumption of development upon hydration, the possible role of the enzyme, in these processes was investigated. The results revealed enzyme's presence and an increase in its activity with the advancement of cyst development. Further partial purification of the enzyme using a lipoprotein linked-Sepharose CL-4B column (an affinity chromatographic principle) was attempted. The purification of 19.3 fold with a yield of 80{\%} was attained.",
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AB - The occurrence of phosphatidyl choline specific phospholipase C (PC-PLC)(EC 3.1.4.3) which liberates diacylglycerol (DAG), an important second messenger molecule, has been reported for the first time from the cryptobiotic cysts of the fairy shrimp, Streptocephalus dichotomus. Since the cysts undergo a revival of metabolism and resumption of development upon hydration, the possible role of the enzyme, in these processes was investigated. The results revealed enzyme's presence and an increase in its activity with the advancement of cyst development. Further partial purification of the enzyme using a lipoprotein linked-Sepharose CL-4B column (an affinity chromatographic principle) was attempted. The purification of 19.3 fold with a yield of 80% was attained.

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