Identification and localization of food-source microbial nucleic acids inside soil nematodes

Amy M. Treonis, Elizabeth Michelle, Cecilia A. O'Leary, Erin E. Austin, Carolyn B. Marks

Research output: Contribution to journalArticle

Abstract

Microorganisms (e.g., prokaryotes, fungi) are food sources for soil nematodes, but they can also be potential mutualists or pathogens. Understanding the linkages between microorganism and invertebrate diversity in soils requires the ability to distinguish between these microbial roles. We tested the potential of a taxon-specific fluorescent in situ hybridization (FISH) procedure for identifying and localizing microbial rRNA within the bodies of soil nematodes. Our objective was to determine whether the rate of digestion permitted detection and identification of food-source nucleic acids within the nematode digestive system (i.e., pharynges, intestines) before their breakdown. First, using laboratory cultures of Caenorhabditis elegans maintained on Escherichia coli, we were able to localize bacterial rRNA throughout the nematode pharynx with the universal bacterial-probe EUB338, although never in the intestines. Second, we applied the fungal rRNA probe FR1 to Aphelenchus avenae cultured on the fungus Rhizoctonia solani. We were unable to detect fungal rRNA within these nematodes, and it appears that this material may be digested rapidly. Next, we applied our technique to nematodes extracted directly from soils. We were able to localize bacterial rRNA within the pharynges of bacterial-feeding species of nematodes from desert soils. We also localized archaeal rRNA using the probe ARC344. Finally, application of EUB338 to desert soil nematodes revealed the presence of bacteria in the intestines of some nematodes and within the ovary of a single nematode. This technique has great potential for use in understanding the feeding behavior of bacterial-feeding soil nematodes and in studies of nematode:bacterial relationships.

Original languageEnglish (US)
Pages (from-to)2005-2011
Number of pages7
JournalSoil Biology and Biochemistry
Volume42
Issue number11
DOIs
StatePublished - Nov 1 2010
Externally publishedYes

Fingerprint

soil nematodes
nucleic acid
acid soil
acid soils
Nucleic Acids
nucleic acids
nematode
Soil
Nematoda
Food
food
ribosomal RNA
Pharynx
pharynx
Intestines
desert soils
intestines
Fungi
desert soil
Rhizoctonia

Keywords

  • Death Valley
  • Fluorescent in situ hybridization
  • Nematodes
  • Soil food webs
  • Trophic behavior

ASJC Scopus subject areas

  • Microbiology
  • Soil Science

Cite this

Identification and localization of food-source microbial nucleic acids inside soil nematodes. / Treonis, Amy M.; Michelle, Elizabeth; O'Leary, Cecilia A.; Austin, Erin E.; Marks, Carolyn B.

In: Soil Biology and Biochemistry, Vol. 42, No. 11, 01.11.2010, p. 2005-2011.

Research output: Contribution to journalArticle

Treonis, Amy M. ; Michelle, Elizabeth ; O'Leary, Cecilia A. ; Austin, Erin E. ; Marks, Carolyn B. / Identification and localization of food-source microbial nucleic acids inside soil nematodes. In: Soil Biology and Biochemistry. 2010 ; Vol. 42, No. 11. pp. 2005-2011.
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