Identification and characterization of a type II restriction endonuclease, StrI from Streptomyces thermodiastaticus

Siddamadappa Chandrashekaran, Aparna Bhavani Shankar, Padmanabhan Babu, Bindu Diana Paul, Valakunja Nagaraja

Research output: Contribution to journalArticlepeer-review

Abstract

A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5′-C↓TCGAG-3′ as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.

Original languageEnglish (US)
Pages (from-to)273-276
Number of pages4
JournalCurrent Science
Volume77
Issue number2
StatePublished - Jul 25 1999
Externally publishedYes

ASJC Scopus subject areas

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