Identification and characterization of a type II restriction endonuclease, StrI from Streptomyces thermodiastaticus

Siddamadappa Chandrashekaran, Aparna Bhavani Shankar, Padmanabhan Babu, Bindu Paul, Valakunja Nagaraja

Research output: Contribution to journalArticle

Abstract

A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5′-C↓TCGAG-3′ as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.

Original languageEnglish (US)
Pages (from-to)273-276
Number of pages4
JournalCurrent Science
Volume77
Issue number2
StatePublished - Jul 25 1999
Externally publishedYes

Fingerprint

Streptomyces thermodiastaticus
Type II Site Specific Deoxyribonucleases
restriction endonucleases
Streptomyces
Enzymes
enzymes
cations
chromatography
enzyme activity
Cations
Chromatography
DNA
temperature
Temperature

ASJC Scopus subject areas

  • General
  • Agricultural and Biological Sciences(all)

Cite this

Identification and characterization of a type II restriction endonuclease, StrI from Streptomyces thermodiastaticus. / Chandrashekaran, Siddamadappa; Shankar, Aparna Bhavani; Babu, Padmanabhan; Paul, Bindu; Nagaraja, Valakunja.

In: Current Science, Vol. 77, No. 2, 25.07.1999, p. 273-276.

Research output: Contribution to journalArticle

Chandrashekaran, S, Shankar, AB, Babu, P, Paul, B & Nagaraja, V 1999, 'Identification and characterization of a type II restriction endonuclease, StrI from Streptomyces thermodiastaticus', Current Science, vol. 77, no. 2, pp. 273-276.
Chandrashekaran, Siddamadappa ; Shankar, Aparna Bhavani ; Babu, Padmanabhan ; Paul, Bindu ; Nagaraja, Valakunja. / Identification and characterization of a type II restriction endonuclease, StrI from Streptomyces thermodiastaticus. In: Current Science. 1999 ; Vol. 77, No. 2. pp. 273-276.
@article{04d8941e323a45459d05beec73a24d38,
title = "Identification and characterization of a type II restriction endonuclease, StrI from Streptomyces thermodiastaticus",
abstract = "A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5′-C↓TCGAG-3′ as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.",
author = "Siddamadappa Chandrashekaran and Shankar, {Aparna Bhavani} and Padmanabhan Babu and Bindu Paul and Valakunja Nagaraja",
year = "1999",
month = "7",
day = "25",
language = "English (US)",
volume = "77",
pages = "273--276",
journal = "Current Science",
issn = "0011-3891",
publisher = "Indian Academy of Sciences",
number = "2",

}

TY - JOUR

T1 - Identification and characterization of a type II restriction endonuclease, StrI from Streptomyces thermodiastaticus

AU - Chandrashekaran, Siddamadappa

AU - Shankar, Aparna Bhavani

AU - Babu, Padmanabhan

AU - Paul, Bindu

AU - Nagaraja, Valakunja

PY - 1999/7/25

Y1 - 1999/7/25

N2 - A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5′-C↓TCGAG-3′ as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.

AB - A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5′-C↓TCGAG-3′ as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.

UR - http://www.scopus.com/inward/record.url?scp=0041782201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0041782201&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0041782201

VL - 77

SP - 273

EP - 276

JO - Current Science

JF - Current Science

SN - 0011-3891

IS - 2

ER -