Identification and characterization of a type II restriction endonuclease, StrI from Streptomyces thermodiastaticus

Siddamadappa Chandrashekaran; Aparna Bhavani Shankar; Padmanabhan Babu; Bindu Diana Paul; Valakunja Nagaraja

Identification and characterization of a type II restriction endonuclease, StrI from Streptomyces thermodiastaticus

Siddamadappa Chandrashekaran, Aparna Bhavani Shankar, Padmanabhan Babu, Bindu Diana Paul, Valakunja Nagaraja

Research output: Contribution to journal › Article › peer-review

Abstract

A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5′-C↓TCGAG-3′ as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.

Original language	English (US)
Pages (from-to)	273-276
Number of pages	4
Journal	Current Science
Volume	77
Issue number	2
State	Published - Jul 25 1999
Externally published	Yes

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title = "Identification and characterization of a type II restriction endonuclease, StrI from Streptomyces thermodiastaticus",

abstract = "A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5′-C↓TCGAG-3′ as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.",

author = "Siddamadappa Chandrashekaran and Shankar, {Aparna Bhavani} and Padmanabhan Babu and Paul, {Bindu Diana} and Valakunja Nagaraja",

year = "1999",

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AU - Chandrashekaran, Siddamadappa

AU - Shankar, Aparna Bhavani

AU - Babu, Padmanabhan

AU - Paul, Bindu Diana

AU - Nagaraja, Valakunja

PY - 1999/7/25

Y1 - 1999/7/25

N2 - A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5′-C↓TCGAG-3′ as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.

AB - A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5′-C↓TCGAG-3′ as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.

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M3 - Article

AN - SCOPUS:0041782201

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ER -

Identification and characterization of a type II restriction endonuclease, StrI from Streptomyces thermodiastaticus

Abstract

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