A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5′-C↓TCGAG-3′ as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.
|Original language||English (US)|
|Number of pages||4|
|State||Published - Jul 25 1999|
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