Identification and characterization of a novel peritrophic matrix protein, Ae-Aper50, and the microvillar membrane protein, AEG12, from the mosquito, Aedes aegypti

Li Shao, Martin Devenport, Hisashi Fujioka, Anil Ghosh, Marcelo Jacobs-Lorena

Research output: Contribution to journalArticlepeer-review

Abstract

Immuno-screening of an adult Aedes aegypti midgut cDNA expression library with anti-peritrophic matrix antibodies identified cDNAs encoding a novel peritrophic matrix protein, termed Ae. aegypti Adult Peritrophin 50 (Ae-Aper50), and the epithelial cell-surface membrane protein, AEG12. Both genes are expressed exclusively in the midguts of adult female mosquitoes and their expression is strongly induced by blood feeding. Ae-Aper50 has a predicted secretory signal peptide and five chitin-binding domains with intervening mucin-like domains. Localization of Ae-Aper50 to the peritrophic matrix was demonstrated by immuno-electron microscopy. Recombinant Ae-Aper50 expressed in baculovirus-infected insect cells binds chitin in vitro. Site-directed mutagenesis was used to study the role that cysteine residues from a single chitin-binding domain play in the binding to a chitin substrate. Most of the cysteine residues proved to be critical for binding. AEG12 has a putative secretory signal peptide at the amino-terminus and a putative glycosyl-phosphatidylinositol (GPI) anchor signal at its carboxyl-terminus and the protein was localized by immuno-electron microscopy to the midgut epithelial cell microvilli.

Original languageEnglish (US)
Pages (from-to)947-959
Number of pages13
JournalInsect Biochemistry and Molecular Biology
Volume35
Issue number9
DOIs
StatePublished - Sep 1 2005

Keywords

  • Aedes aegypti
  • Microvilli
  • Midgut
  • Mosquito
  • Peritrophic matrix

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Insect Science

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