Identification and Characterization of a Novel Inositol Hexakisphosphate Kinase

Adolfo Saiardi, Eiichiro Nagata, Hongbo R. Luo, Adele M Snowman, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

The inositol pyrophosphate disphosphoinositol pentakisphosphate (PP-InsP3/InsP7) is formed in mammals by two recently cloned inositol hexakiphosphate kinases, InsP6K1 and InsP 6K2 (Saiardi, A., Erdjument-Bromage, H., Snowman, A. M., Tempst, P., and Snyder, S. H. (1999) Curr. Biol. 9, 1323-1326). We now report the identification, cloning, and characterization of a third InsP7 forming enzyme designated InsP6K3. InsP6K3 displays 50 and 45% sequence identity to InsP6K1 and InsP6K2, respectively, with a smaller mass (46 kDa) and a more basic character than the other two enzymes. InsP6K3 is most enriched in the brain where its localization resembles InsP6K1 and InsP6K2. Intracellular disposition discriminates the three enzymes with InsP6K2 being exclusively nuclear, InsP6K3 predominating in the cytoplasm, and InsP6K1 displaying comparable nuclear and cytosolic densities.

Original languageEnglish (US)
Pages (from-to)39179-39185
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number42
DOIs
StatePublished - Oct 19 2001

Fingerprint

Inositol
Enzymes
Mammals
Cloning
Organism Cloning
Brain
Cytoplasm
Phosphotransferases
Display devices
inositol hexakisphosphate kinase
inositol heptakisphosphate
diphosphoric acid

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification and Characterization of a Novel Inositol Hexakisphosphate Kinase. / Saiardi, Adolfo; Nagata, Eiichiro; Luo, Hongbo R.; Snowman, Adele M; Snyder, Solomon H.

In: Journal of Biological Chemistry, Vol. 276, No. 42, 19.10.2001, p. 39179-39185.

Research output: Contribution to journalArticle

Saiardi, Adolfo ; Nagata, Eiichiro ; Luo, Hongbo R. ; Snowman, Adele M ; Snyder, Solomon H. / Identification and Characterization of a Novel Inositol Hexakisphosphate Kinase. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 42. pp. 39179-39185.
@article{88c41c13b147496fb1bcc2d306c78447,
title = "Identification and Characterization of a Novel Inositol Hexakisphosphate Kinase",
abstract = "The inositol pyrophosphate disphosphoinositol pentakisphosphate (PP-InsP3/InsP7) is formed in mammals by two recently cloned inositol hexakiphosphate kinases, InsP6K1 and InsP 6K2 (Saiardi, A., Erdjument-Bromage, H., Snowman, A. M., Tempst, P., and Snyder, S. H. (1999) Curr. Biol. 9, 1323-1326). We now report the identification, cloning, and characterization of a third InsP7 forming enzyme designated InsP6K3. InsP6K3 displays 50 and 45{\%} sequence identity to InsP6K1 and InsP6K2, respectively, with a smaller mass (46 kDa) and a more basic character than the other two enzymes. InsP6K3 is most enriched in the brain where its localization resembles InsP6K1 and InsP6K2. Intracellular disposition discriminates the three enzymes with InsP6K2 being exclusively nuclear, InsP6K3 predominating in the cytoplasm, and InsP6K1 displaying comparable nuclear and cytosolic densities.",
author = "Adolfo Saiardi and Eiichiro Nagata and Luo, {Hongbo R.} and Snowman, {Adele M} and Snyder, {Solomon H}",
year = "2001",
month = "10",
day = "19",
doi = "10.1074/jbc.M106842200",
language = "English (US)",
volume = "276",
pages = "39179--39185",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "42",

}

TY - JOUR

T1 - Identification and Characterization of a Novel Inositol Hexakisphosphate Kinase

AU - Saiardi, Adolfo

AU - Nagata, Eiichiro

AU - Luo, Hongbo R.

AU - Snowman, Adele M

AU - Snyder, Solomon H

PY - 2001/10/19

Y1 - 2001/10/19

N2 - The inositol pyrophosphate disphosphoinositol pentakisphosphate (PP-InsP3/InsP7) is formed in mammals by two recently cloned inositol hexakiphosphate kinases, InsP6K1 and InsP 6K2 (Saiardi, A., Erdjument-Bromage, H., Snowman, A. M., Tempst, P., and Snyder, S. H. (1999) Curr. Biol. 9, 1323-1326). We now report the identification, cloning, and characterization of a third InsP7 forming enzyme designated InsP6K3. InsP6K3 displays 50 and 45% sequence identity to InsP6K1 and InsP6K2, respectively, with a smaller mass (46 kDa) and a more basic character than the other two enzymes. InsP6K3 is most enriched in the brain where its localization resembles InsP6K1 and InsP6K2. Intracellular disposition discriminates the three enzymes with InsP6K2 being exclusively nuclear, InsP6K3 predominating in the cytoplasm, and InsP6K1 displaying comparable nuclear and cytosolic densities.

AB - The inositol pyrophosphate disphosphoinositol pentakisphosphate (PP-InsP3/InsP7) is formed in mammals by two recently cloned inositol hexakiphosphate kinases, InsP6K1 and InsP 6K2 (Saiardi, A., Erdjument-Bromage, H., Snowman, A. M., Tempst, P., and Snyder, S. H. (1999) Curr. Biol. 9, 1323-1326). We now report the identification, cloning, and characterization of a third InsP7 forming enzyme designated InsP6K3. InsP6K3 displays 50 and 45% sequence identity to InsP6K1 and InsP6K2, respectively, with a smaller mass (46 kDa) and a more basic character than the other two enzymes. InsP6K3 is most enriched in the brain where its localization resembles InsP6K1 and InsP6K2. Intracellular disposition discriminates the three enzymes with InsP6K2 being exclusively nuclear, InsP6K3 predominating in the cytoplasm, and InsP6K1 displaying comparable nuclear and cytosolic densities.

UR - http://www.scopus.com/inward/record.url?scp=0035914367&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035914367&partnerID=8YFLogxK

U2 - 10.1074/jbc.M106842200

DO - 10.1074/jbc.M106842200

M3 - Article

C2 - 11502751

AN - SCOPUS:0035914367

VL - 276

SP - 39179

EP - 39185

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 42

ER -