Identification and Characterization of a Novel Inositol Hexakisphosphate Kinase

Adolfo Saiardi, Eiichiro Nagata, Hongbo R. Luo, Adele M. Snowman, Solomon H. Snyder

Research output: Contribution to journalArticlepeer-review

Abstract

The inositol pyrophosphate disphosphoinositol pentakisphosphate (PP-InsP3/InsP7) is formed in mammals by two recently cloned inositol hexakiphosphate kinases, InsP6K1 and InsP 6K2 (Saiardi, A., Erdjument-Bromage, H., Snowman, A. M., Tempst, P., and Snyder, S. H. (1999) Curr. Biol. 9, 1323-1326). We now report the identification, cloning, and characterization of a third InsP7 forming enzyme designated InsP6K3. InsP6K3 displays 50 and 45% sequence identity to InsP6K1 and InsP6K2, respectively, with a smaller mass (46 kDa) and a more basic character than the other two enzymes. InsP6K3 is most enriched in the brain where its localization resembles InsP6K1 and InsP6K2. Intracellular disposition discriminates the three enzymes with InsP6K2 being exclusively nuclear, InsP6K3 predominating in the cytoplasm, and InsP6K1 displaying comparable nuclear and cytosolic densities.

Original languageEnglish (US)
Pages (from-to)39179-39185
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number42
DOIs
StatePublished - Oct 19 2001

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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