Identification and characterization of a conserved family of protein serine/threonine phosphatases homologous to Drosophila retinal degeneration C (rdgC)

Patti M. Sherman, Hui Sun, Jennifer P. Macke, John Williams, Philip M. Smallwood, Jeremy Nathans

Research output: Contribution to journalArticle

Abstract

The Drosophila retinal degeneration C (rdgC) gene encodes an unusual protein serine/threonine phosphatase in that it contains at least two EF- hand motifs at its carboxy terminus. By a combination of large-scale sequencing of human retina cDNA clones and searches of expressed sequence tag and genomic DNA databases, we have identified two sequences in mammals [Protein Phosphatase with EF-hands-1 and 2 (PPEF-1 and PPEF-2)] and one in Caenorhabditis elegans (PPEF) that closely resemble rdgC. In the adult, PPEF- 2 is expressed specifically in retinal rod photoreceptors and the pineal. In the retina, several isoforms of PPEF-2 are predicted to arise from differential splicing. The isoform that most closely resembles rdgC is localized to rod inner segments. Together with the recently described localization of PPEF-1 transcripts to primary somatosensory neurons and inner ear cells in the developing mouse, these data suggest that the PPEF family of protein serine/threonine phosphatases plays a specific and conserved role in diverse sensory neurons.

Original languageEnglish (US)
Pages (from-to)11639-11644
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number21
DOIs
StatePublished - Oct 14 1997

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Retinal Degeneration
Phosphoprotein Phosphatases
Drosophila
EF Hand Motifs
Retina
Protein Isoforms
Retinal Photoreceptor Cell Inner Segment
Peptide Elongation Factor 2
Peptide Elongation Factor 1
Retinal Rod Photoreceptor Cells
Expressed Sequence Tags
Nucleic Acid Databases
Caenorhabditis elegans
Sensory Receptor Cells
Inner Ear
Mammals
Complementary DNA
Clone Cells
Neurons
Genes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Identification and characterization of a conserved family of protein serine/threonine phosphatases homologous to Drosophila retinal degeneration C (rdgC). / Sherman, Patti M.; Sun, Hui; Macke, Jennifer P.; Williams, John; Smallwood, Philip M.; Nathans, Jeremy.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 21, 14.10.1997, p. 11639-11644.

Research output: Contribution to journalArticle

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