Identification and analysis of a lytic-phase origin of DNA replication in human herpesvirus 7

N. Van Loon, C. Dykes, H. Deng, G. Dominguez, J. Nicholas, S. Dewhurst

Research output: Contribution to journalArticlepeer-review

Abstract

Human herpesvirus 7 (HHV-7) DNA sequences colinear with the HHV-6 lytic- phase origin of DNA replication (oriLyt) were amplified by PCR. Plasmid constructs containing these sequences were replicated in HHV-7-infected cord blood mononuclear cells but not in HHV-6-infected cells. In contrast, plasmids bearing HHV-6 oriLyt were replicated in both HHV-6- and HHV-7- infected cells. Finally, the minimal HHV-7 DNA element necessary for replicator activity was mapped to a 600-bp region which contains two sites with high homology to the consensus binding site for the HHV-6 origin binding protein. At least one of these binding sites was shown to be essential for replicator function of HHV-7 oriLyt.

Original languageEnglish (US)
Pages (from-to)3279-3284
Number of pages6
JournalJournal of virology
Volume71
Issue number4
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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