IκB kinases serve as a target of CD28 signaling

Edward W. Harhaj, Shao Cong Sun

Research output: Contribution to journalArticle

Abstract

Optimal T cell activation and interleukin-2 production requires a second signal in addition to antigen-mediated T cell receptor (TCR) signaling. The CD28 molecule has been demonstrated to act as an effective costimulatory molecule upon binding by B7.1 or B7.2 present on antigen-presenting cells. The CD28 signal acts in concert with the TCR signal to significantly augment activation of the NF-κ family of transcription factors. The interleukin-2 gene is regulated by NF-κB among other transcription factors, in part, via a CD28 responsive element (CD28RE) present in the IL-2 promoter. Enhanced activation of NF-κB by CD28 is mediated by rapid phosphorylation and proteasome-mediated degradation of the NF-κB inhibitory proteins IκBα and IκBβ, which allows for accelerated nuclear expression of the liberated NF- κB. Herein, we provide evidence that the catalytic activities of two recently identified IκB kinases, IKKα and IKKβ, are significantly elevated when T cells are stimulated through CD28 in addition to mitogen treatment. Catalytically inactive forms of IKKs are able to block the in vivo phosphorylation of IκBα induced by mitogen and CD28. Furthermore, CD28- mediated reporter gene transactivation of the CD28RE/AP-1 composite element is consistently attenuated by the IKK mutants. These findings suggest that cellular signaling pathways initiated at the TCR and CD28 converge at or upstream of IKK, resulting in more robust kinase activity and enhanced and prolonged NF-κB activation.

Original languageEnglish (US)
Pages (from-to)25185-25190
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number39
DOIs
StatePublished - Sep 25 1998
Externally publishedYes

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T-Cell Antigen Receptor
Interleukin-2
Phosphotransferases
Chemical activation
Mitogens
Phosphorylation
T-cells
Transcription Factors
T-Lymphocytes
Genes
Transcription Factor AP-1
Antigen-Presenting Cells
Proteasome Endopeptidase Complex
Cell signaling
Reporter Genes
Transcriptional Activation
Molecules
Catalyst activity
Antigens
Degradation

ASJC Scopus subject areas

  • Biochemistry

Cite this

IκB kinases serve as a target of CD28 signaling. / Harhaj, Edward W.; Sun, Shao Cong.

In: Journal of Biological Chemistry, Vol. 273, No. 39, 25.09.1998, p. 25185-25190.

Research output: Contribution to journalArticle

Harhaj, Edward W. ; Sun, Shao Cong. / IκB kinases serve as a target of CD28 signaling. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 39. pp. 25185-25190.
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